Role of Nrf2 pathway in flutamide-induced mitochondrial biogenesis
10.12092/j.issn.1009-2501.2022.05.002
- Author:
Li ZHANG
1
;
Hui PENG
1
;
Jun HE
1
;
Shuangqing PENG
1
;
Jiabin GUO
1
;
Jin WANG
2
;
Huizi LI
2
Author Information
1. Chinese PLA Center for Disease Control and Prevention
2. Chinese PLA Rocket Force Characteristic Medical Center
- Publication Type:Journal Article
- Keywords:
ERK1/2;
Flutamide;
Mitochondrial biogenesis;
Nrf2;
PGC-1α
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2022;27(5):498-504
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of flutamide on mitochondrial biogenesis and the regulating effect of anoxidative pathway Nrf2 on it. METHODS: Human hepatocyte HepG2 cells were treated with flutamide (0-50 μmol/L) for 24 h, then mtDNA copy number and protein expression of mitochondrial biogenesis were detected by RT-PCR and WB. The effects of ERK1/2 and the role of Nrf2 pathway in mitochondrial biogenesis were further observed by gene knockdown and protein activation/inhibition methods. RESULTS: Flutamide interfered mitochondrial biogenesis concentration-dependently, the mtDNA copy number, ERK1/2 and PGC-1α proteins increased with the dose. ERK1/2 inhibition and activation regulated flutamide-induced mtDNA copy number and PGC-1α expression, and inhibition of Nrf2 pathway also affected flutamide-induced mtDNA copy number and expression of PGC-1α, as well as ERK1/2 expression. CONCLUSION: Flutamide affects mitochondrial biogenesis, and the antioxidant pathway Nrf2 may be involved in the regulation of flutamine-induced mitochondrial biogenesis by regulating ERK1/2.
