Involvement of autophagy in iron ion regulation promotes ferroptosis in cells undergoing intestinal ischemia-reperfusion injury
10.12092/j.issn.1009-2501.2023.01.005
- Author:
Jingyu ZHANG
1
;
Yihan WANG
1
;
Jun LI
1
;
Ping JIN
1
;
Yingbin WANG
1
;
Jieting LIU
1
;
Xiping SHEN
2
Author Information
1. Department of Anesthesiology, Second Hospital of Lanzhou University
2. Institute of Epidemiology and Health Statistics, School of Public Health, Lanzhou University
- Publication Type:Journal Article
- Keywords:
autophagy;
ferroptosis;
intestinal ischemia;
reperfusion
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2023;28(1):36-41
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of autophagy on cell ferroptosis in intestinal ischemia-reperfusion injury. METHODS: Twenty-four SPF grade Wistar rats weighing 200-220 g were divided into 4 groups (n = 6): sham operation group (sham group), ischemia group (I group), ischemia-reperfusion group (I/R group),and ischemia-reperfusion + autophagy inhibitor group (I/R + 3-MA group). The ischemia model was established by clamping the superior mesenteric artery for 1 hour, and the intestinal ischemia-reperfusion injury model was established by reperfusion for 2 hours. HE staining was used to observe the pathological changes of intestinal mucosa and Chiu score under light microscope. Fe
