Effect of Atglistatin on PA-induced VSMCs proliferation and ATGL expression
10.12092/j.issn.1009-2501.2023.01.004
- Author:
Zizhao TANG
1
;
Fangqin NIE
1
;
Xin YAN
1
;
Yangxia FU
1
;
Jun HUANG
1
;
Xia WANG
1
;
Ren GUO
1
Author Information
1. The Third Xiangya Hospital, Central South University
- Publication Type:Journal Article
- Keywords:
adipose triglyceride lipase;
arteriosclerosis;
Atglistatin;
lipid droplet;
palmitic acid
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2023;28(1):29-35
- CountryChina
- Language:Chinese
-
Abstract:
AIM: With building a proliferation model of PA-induced VSMC, the effect of ATGL, a key fat metabolism enzyme, on the phenotype transformation of VSMC was preliminarily explored. METHODS: 40 μmol/L Atglistatin was added to the proliferation model of VSMC induced by PA (50 μmol/L, 100 μmol/L, and 200 μmol/L, respectively) at separately administered concentrations, and cell viability and cell proliferation were detected by CCK-8 and EDU; cell migration ability was detected by scratch assay; oil red staining was used to detect the accumulation of lipid droplets in VSMC was detected by oil red staining; the effects of PA on ATGL as well as the effects of smooth muscle contraction phenotype proteins were examined by Western blot. RESULTS: PA at a concentration of 100 μmol/L could significantly induce VSMC proliferation, promote lipophagy and increase lipid droplet accumulation in VSMC; meanwhile, Atglistatin could exacerbate these changes caused by PA and increase lipid droplet accumulation in VSMC. CONCLUSION: Atglistatin exacerbates PA-induced VSMC proliferation and increases VSMC lipid droplet accumulation, and exacerbates transformation of proliferative phenotype of VSMC.
