Resveratrol inhibits advanced glycation end products induced PC12 cell apoptosis through ROS-JNK pathway
10.3969/j.issn.1001-1978.2021.08.013
- Author:
Lei JIANG
1
;
Guo-Jun ZHANG
1
;
Lei JIANG
2
;
Guo-Jun ZHANG
2
;
Lei JIANG
3
;
Rui ZHANG
3
;
Shun-Jiang XU
3
;
Yan-Chao LU
4
;
Jia-Kai ZHANG
5
;
Guo-Jun ZHANG
6
Author Information
1. Laboratory Diagnosis Center, Beijing Tiantan Hospital Affiliated to Capital Medical University
2. Beijing Engineering Research Center of Immunological Reagents Clinical Research
3. Central Laboratory, the First Hospital of Hebei Medical University
4. the First Hospital of Hebei Medical University, Dept of Nuclear Medicine
5. the First Hospital of Hebei Medical University, Dept of Radiology
6. NMPA Key Laboratory for Quality Control of in Vitro Diagnostics
- Publication Type:Journal Article
- Keywords:
advanced glycation end products;
apoptosis;
JNK specific phosphorylation inhibitor (sp600125);
PC 12 cells;
resveratrol;
ROS-JNK signaling pathway
- From:
Chinese Pharmacological Bulletin
2021;37(8):1104-1109
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of resveratrol (RSV)on apoptosis of PC 12 cells induced by advanced glycation end products(AGEs) and its possible molecular mechanism. Methods MTS assay was used to detect the effects of AGEs (0, 50, 100, 200, 400 g • L"1) and RSV (0, 5, 10, 20, 40 jxmol • L"1) on cell viability. PC12 cells were treated with AGEs (400 g • L-1) and RSV (10 |xmol • L"1). TUNEL staining was used to detect apoptosis; flow cytometry was used to detect apoptosis and reactive oxygen species (ROS) ; Western blot was used to detect protein expression of p-JNK, JNK, PUMA, Bcl-2, Bax and Caspase-3. Apoptosis was observed by flow cytometry after pretreat- ment with JNK specific phosphorylation inhibitor (sp600125). Results Compared with normal control group, the cell viability of AGEs group decreased, the apoptotic rate and ROS levels increased, the expressions of p-JNK, PUMA, Bax and Caspase-3 protein in-creased, the expression of Bcl-2 protein decreased; Compared with AGEs group, the cell viability of the AGEs + RSV group increased, the levels of apoptotic rate and ROS were reduced, the expressions of p-JNK, PUMA, Bax and caspase-3 protein decreased, the expression of Bcl-2 protein increased. Sp600125 could partially reverse the effect of AGEs on PC)2 cell apopto-sis. Conclusions RSV can significantly inhibit the apoptosis of PC 12 cells induced by AGEs, which may be related to the activation of ROS-JNK pathway.
