The protective effect of hesperidin on injury induced by glucolipotoxicity in islet beta cells
10.3969/j.issn.1001-1978.2021.05.011
- Author:
Yun-Qi ZHANG
1
;
Xiao-Jing LIN
1
;
Miao-Ran WANG
1
;
Yue LI
1
;
Lei FAN
1
;
Xiao-Qiu XIAO
1
;
Yun-Qi ZHANG
2
;
Yi HOU
3
Author Information
1. The Chongqing Key Laboratory of Translationsl Medicine in Major Metabolic Diseases, The First Affiliated Hospital of Chongqing Medical University, Chongqing Medical University
2. College of Pharmacy, Chongqing Medical University
3. The Experimental Teaching Management Center of Traditional Chinese Medicine Laboratory, Chongqing Medical University
- Publication Type:Journal Article
- Keywords:
apoptosis;
diabetes;
hesperidin;
pancreas;
pancreatic β-cells;
proliferation
- From:
Chinese Pharmacological Bulletin
2021;37(5):652-656
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the protective effect of hesperidin (HSD) on the injury of mouse pancreatic beta cells induced by high glucose and fatty acid and the underlying mechanism. Methods MIN6 cells were treated with high glucose and fatty acid after pretreatment of HSD. Cell counting kit-8 (CCK-8) and Hoechst 33258 fluorescence staining were used to determine the proliferation and apoptosis of MIN6 cells. Western blot was used to detect the expressions of apoptosis-related proteins Bcl-2 and Bax. RT-PCR was used to detect the expressions of inflammatory factors tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β). ELISA was used to test the insulin secretion of pancreatic islets. Results High glucose and fatty acid decreased the ratio of Bcl-2/Bax, increased the expression of inflammatory factors TNF-α and IL-1β and inhibited the insulin secretion of mouse pancreatic islets. After pretreatment of HSD, the cell viability and Bcl-2/Bax ratio of MIN6 increased, the expressions of inflammatory factors TNF-α and IL-1β decreased, and the insulin secretion of mouse pancreatic islets increased. Conclusions HSD could resist the apoptosis of mouse pancreatic islet B cell line MIN6 induced by high fat and high glucose, reduce the secretion of inflammatory factors and improve the insulin secretion of pancreatic islets.
