Effects of 5-aza-2'-deoxycytidine on proliferation of SPC-Al cells and expression of SFRPl and MGMT methylated proteins in lung cancer
10.3969/j.issn.1001-1978.2021.03.013
- Author:
Cong-Shu HUA
1
;
Hai CHEN
1
;
Teng-Fei GE
1
;
Jing YE
2
;
Xiao-Yu CHEN
3
Author Information
1. Dept of Thoracic Surgery, Chest Clinical Hospital of Anhui Medical University, Anhui Province Chest Hospital
2. Dept of Respiratory Medicine, the Second Hospital of Anhui Medical University
3. Experimental Center of Morphology, Anhui Medical University
- Publication Type:Journal Article
- Keywords:
06-methylguanine-DNA-methyltransferase;
5-aza-2'-de-oxycytidine;
Apoptosis;
Cell proliferation;
Lung cancer SPC-A 1 cells;
Secreted frizzled related protein 1
- From:
Chinese Pharmacological Bulletin
2021;37(3):367-371
- CountryChina
- Language:Chinese
-
Abstract:
Aim To observe the effect of methylation inhibitors of 5-aza-2'-deoxycytidine (5-Aza-CdR) on SPC-Al - lung cancer cell proliferation, cell scratches and apoptosis, to explore the influence of secretion curl associated protein 1 (SFRPl) and 06-methylguanine-DNA-methyltransferase (MGMT) gene promoter region in which DNA methylation mRNA and protein expression and meaning. Methods The effects of 5-Aza-CdR at different concentrations on the proliferation of human lung cancer SPC-A 1 cells were determined by CCK-8 assay. The effect of 5-Aza-CdR on the migration ability of SPC-A 1 cells was determined by scratch assay. The apoptosis of lung cancer SPC-A 1 cells was detected by Hoechst 33258 staining after treatment with 5-Aza-CdR for 24 h. mRNA and protein expressions of SFRPl and MGMT in SPC-Al cells were detected by RT-PCR and Western blot. Results 5-Aza-CdR could reduce the proliferation of SPC-A 1 cells by concentration gradient,and IC50 was 21.2 jjimol • L
