Study on anti-inflammatory effect of Salvia miltiorrhiza-Kushen herbpairs extract based on network pharmacology
10.3969/j.issn.1001-1978.2021.02.022
- Author:
Peng CHENG
1
;
Shuai HUANG
1
;
Yu YANG
1
;
Jia-Wei WU
1
;
Wen-Xing CHEN
1
;
Ai-Yun WANG
1
;
Yin LU
1
Author Information
1. Jiangsu Key Lab for Pharmacology and Safety Evaluation of Chinese Materia Medica, School of Pharmacy, Nanjing University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
ear edema model;
G-CSF;
interleukin-6;
network pharmacology;
Salvia miltiorrhiza-Kushenherb pairs;
vascular permeability
- From:
Chinese Pharmacological Bulletin
2021;37(2):270-277
- CountryChina
- Language:Chinese
-
Abstract:
Aim To predict the potential targets of Salvia miltiorrhiza- Kushen herb pairs extracts (SK) and explore its anti-inflammatory effect on modelsbased on network pharmacology, so as to provide the research foundation of both new drugs and anti-inflammatory mechanism. Methods Network pharmacology was used to predict the potential anti-inflammatory targets of SK. Ear edema model was used to study the anti-in- flammatoiy effects of SK. Luminex liquid-phase chip analysis technology was used to observethe changes in secretion of pro-inflammatory cytokines in peripheral serumafter transdermal administration in mice by SK. Intraperitoneal injection of Evans blue experiment was used to simulate the exudation of inflammatory factors, and the effect of SK on capillary permeability in mice- was explored. Results Network pharmacology was used to predict that the anti-inflammatory effect of SK- was mainly related to immune-related processes and VEGF signaling pathways, unravelingthe relationship between the anti-inflammatory mechanismand the decrease of pro-inflammatory factors and vascular permea- bility. Conclusions The experiments verify that the results are the same as the prediction by network pharmacology. The anti-inflammatory effect involves decline the IL-6 levels, granulocyte colony-stimulating factor( G-CSF) levels and vascular permeability in the STAT3 pathway. Asthemain components of SK, tanshi- none, matrine and oxymatrine are linked to anti-in- flammatory effects.
