Feasibility study on BN rats and RBL-2H3 cells as models of allergic reaction to Yin Zhihuang injection

Author: Yu ZHU ¹ ; Jun-Xia ZHANG ² ; Ji-Chun ZHOU ² ; Zhan LI ² ; Zhen-Guo LI ²
Author Information

1. Dept of Pharmacy, Henan Province Hospital of Traditional Chinese Medicine
2. Dept of Pharmacology, Henan Provincial Institute of Food and Drug Control
Publication Type:Journal Article
Keywords: allergic reaction; BN rats; Ca; histamine; RBL-2H3 cells; Yin zhihuang injection
From: Chinese Pharmacological Bulletin 2022;38(12):1908-1914
CountryChina
Language:Chinese
Abstract: Aim To explore the establishment of BN rat animal model and RBL-2H3 cell model of allergic reaction to Yinzhihuang injection. Methods ASA test was performed to compare the symptoms and grades of allergic reactions in BN rats and Hartley guinea pigs, and serum IgE and histamine levels were detected. The amount of histamine released from RBL-2H3 cell supernatant was measured after Yinzhihuang injection affected, and the intracellular Ca