Intermedin inhibits lipopolysaccharide-induced polarization of macrophages by activating AMPK signaling pathway
10.3969/j.issn.1001-1978.2022.02.009
- Author:
Y An-Hong WANG
1
;
Ji-Hua TIAN
1
;
Jia YANG
1
;
Yuan XUE
1
;
Ting-Ting ZHANG
1
;
Pei-Xia YU
2
;
Pei-Xia YU
3
Author Information
1. Shanxi Medical University, Dept of Microbiology and Immunology
2. Dept of Clinical Laboratory, Third Hospital of Shanxi Medical University, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences
3. Tongji Medical College, Huazhong University of Science and Technology, Tongji Hospital
- Publication Type:Journal Article
- Keywords:
AMPK signaling pathway;
inflammation;
intermedin;
LPS;
M1/M2 polarization;
macrophage
- From:
Chinese Pharmacological Bulletin
2022;38(2):196-201
- CountryChina
- Language:Chinese
-
Abstract:
Aim To evaluate the mechanism by which intermdin(IMD)inhibits lipopolysaccha ride(LPS)-induced polarization in RAW264.7 cells.Methods RAW264.7 cells were divided into control groups, LPS groups, LPS+IMD groups, LPS+IMD+Compound C groups.The mRNA expressions of tumor necrosis factor-α,(TNF-α,), CD86, inducible nitric oxide synthase(iNOS), Arginase-1(Arg-1)and CD206 were detected by Realtime-PCR.The protein expressions of p-AMPK, AMPK, TNF-α, intereukin-6(IL-6)and intereukin-10(IL-10)were detected by Western blot.The proportion of CD86+ M1 type cells was detected by Flow cytometry.In addition, the expression levels of supernatant cytokines, including IL-6 and TNF-α, were detected by ELISA.Results Compared with control and LPS groups, IMD treatment could up-regulate the expression level of p-AMPK and the ratio of p-AMPK/AMPK.LPS promoted M1 polarization, since the expressions of CD86, TNF-α and iNOS increased, while the expressions of CD206 and Arg-1 decreased by LPS induction.The proportion of M1 type cells increased and the secretion of TNF-α, IL-6 in the cell supernatant increased.And IMD treatment could inhibit the polarization of M1 induced by LPS.These effects were reversed by Compound C, an inhibitor of AMPK.Conclusion IMD can inhibit the M1-type polarization of LPS-induced macrophages by activating AMPK signaling pathway.
