Research on mechanism of salidroside activating HIF-1 a signaling pathway to promote MOB proliferation
10.3969/j.issn.1001-1978.2022.02.008
- Author:
Yu-Tong JIN
1
;
Xin-Rui NIU
1
;
Ji-Da WANG
1
;
Shuang YU
1
;
Jia-Ying LI
1
;
Yu-Hong BIAN
1
;
Yue WANG
1
;
Lin QI
2
Author Information
1. School of Integrative Medicine, Tianjin University of Traditional Chinese Medicine
2. Characteristic Medical Center of Pap, Dept of Pharmacy
- Publication Type:Journal Article
- Keywords:
angiogenesis;
bone formation;
hypoxia-inducible factor-la;
osteoblasts;
proliferation;
salidroside
- From:
Chinese Pharmacological Bulletin
2022;38(2):190-195
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the effeet of salidroside (SAL) on proliferation of osteoblasts and its possible mechanism by using mouse primary osteoblasts ( MOB) as the research object.Methods Alkaline phosphatase ( ALP) staining was used to identify the extracted primary cells.MTT was used to detect the effect of SAL on the proliferation.RT-PCR, Western blot, ELISA were used to investigate the molecular mechanism of SAL.Results The extracted cells generated black-brown deposits by ALP staining, which were shown to be osteoblasts clearly.SAL promoted the pro liferation of MOB.Meanwhile, SAL could up-regulate the mRNA and protein expression levels of hypoxia-inducible factor-lcx ( HIF-1 ex), vascular endothelial growth factor ( VEGF ) , angiopoietin-like protein 4 (ANGPTL4) and interleukin 6 ( IL-6 ) , which were down-regulated by using the HIF-lex blocker YC-1.Conclusions SAL could promote the proliferation of MOB through HIF-1 a/VEGF, ANGPTL4, IL-6 signaling pathway.
