The protective effects of naringenin on liver fibrosis by regulating TGF-fil/smad pathway
10.3969/j.issn.1001-1978.2022.02.017
- Author:
Shan-Shan YUE
1
;
Shan-Shan YUE
2
;
An-Kang PENG
2
;
Ze-Jiang MA
2
;
Rong QI
2
Author Information
1. School of Basic Medical Science, Shihezi University
2. Peking University Health Science Center, School of Basic Medical Sciences, Dept of Pharmacology
- Publication Type:Journal Article
- Keywords:
hepatic stellate cells;
liver fibrosis;
MCD diet;
naringenin;
TGF-β1;
TGF-β1/smad pathway
- From:
Chinese Pharmacological Bulletin
2022;38(2):248-254
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the effects of naringenin on MCD diet-induced liver fibrosis and its related mechanisms.Methods LX2 cells were incubated with TGF-β1 for 24 h to establish the in vitro fibrosis model.LX2 cells were treated with NGN at the same time.Male C57BL/6 mice were fed with MCD diet for six weeks to induce liver fibrosis.100 mg·kg-1·d-1 NGN was administered by gavage simultaneously.The protein expressions of α-SMA, col1, TGF-β1, p-smad2 and p-smad3 were evaluated by Western blot.The mRNA expressions of α-SMA, col1 and col3 were detected by qRT-PCR.The degree of liver fibrosis was evaluated by Sirius red staining.Results Both in in vivo and in vitro experiments, compared with model group, the mRNA levels of α-SMA, col1 and col3 and protein levels of α-SMA, TGF-β1, p-smad2 and p-smad3 significantly decreased in NGN treatment group.The results of HE staining and Sirius red staining also indicated that NGN significantly decreased liver fibrosis induced by MCD diet.Conclusions Naringin can significantly inhibit liver fibrosis induced by MCD diet, which may be related to TGF-β1/Smad pathway.
