The renal protective effect of saxagliptin combined with metformin on type 2 diabetic mice and its relationship with redox balance
- Author:
Xiao-Ying WU
1
;
Wen-Jie WEN
1
;
Qi-Lun ZHANG
1
;
Jing-Fan XUE
1
;
Wan ZHOU
1
;
Shan-Dong YE
1
Author Information
- Publication Type:Journal Article
- Keywords: diabetic kidney disease; metformin; oxidative stress; red-ox balance; saxa¬gliptin; type 2 diabetes mice
- From: Chinese Pharmacological Bulletin 2022;38(7):1038-1042
- CountryChina
- Language:Chinese
- Abstract: Aim To evaluate the reno-proteetion of saxagliptin combined with metformin in mice with T2DM anrl its relationship with redox balance.Meth¬ods C57BL/6J mice were fed with high fat diet and injected with low dose STZ intraperitoneally to establish T2DM mouse model.Then they were randomly divided into T2DM group, glibenclamide group ( Gli group), metformin group ( Met group) , saxagliptin group ( Sax group) and saxagliptin + metformin group ( S + M group) , and normal control group ( NC group) with 8 mice in each group.Eight weeks after intervention the mice were weighed.Blood, urine and renal tissue sam¬ples were collected to measure GHbA,c, FBG, Alb, 8-OHdG, 8-iso-PG, SOD, GSH, GSSG and Ucr.The pathological morphology of renal tissues in each group was observed.Results Saxagliptin combined with metformin reduced significantly the levels of Alb/Ucr ( UACR) , 8-0HdG/Ucr( UOCR) , 8-iso-PG/Ucr( UP- CR) , increased the activity of SOD and GSH/GSSG ratio, and improved the pathological changes of renal tissues, which were superior to those in Met group and Sax group.Conclusions Saxagliptin combined with metformin have a synergistic protective effect on the kidneys of type 2 diabetic mice.The mechanism is partly related to alleviating oxidative stress and impro¬ving redox balance in vivo.
