The protective effect of lentinan regulating autophagy on HUVEC injury induced by high glucose and its mechanism
- Author:
Qiao-Wei YANG
1
;
Li WU
1
;
Hong-Liang MEI
1
;
Qiao-Wei YANG
2
;
Huan-Yu NI
2
;
Hong-Liang MEI
2
;
Qiao-Wei YANG
3
;
Li WU
3
;
Huan-Yu NI
4
;
Hong-Liang MEI
4
Author Information
- Publication Type:Journal Article
- Keywords: autophagy: ROS; HUVECs; lentinan; oxidative stress; p38 MAPK pathway
- From: Chinese Pharmacological Bulletin 2022;38(8):1182-1189
- CountryChina
- Language:Chinese
- Abstract: Aim To study the effect of lentinan ( LNT) on the injury of human umbilical vein endothelial cells (HUVECs) induced by high concentration of glucose ( HG) and its mechanism so as to provide a new theo¬retical basis for the treatment of diabetic angiopathy.Methods After screening the optimal concentration of HG-induced HUVEC injury, different concentrations of LNT were given and then HUVEC cell viability, reac¬tive oxygen species ( ROS ) , superoxide dismutase (SOD) ,and malondialdehyde (MDA) levels were de¬tected.Autophagy level in HUVECs was determined by MDC staining.Beclin-1 level was detected by PCR.The expression of LC3, inducible nitric oxide synthase (iNOS) and the phosphorylation level of p38 MAPK were detected by Western blot.Results 120 mmol • L"1 HG could cause moderate HUVEC injury.LNT could improve the declining HUVEC viability induced by HG, alleviate the increasing ROS,upgrade the level of SOD level, downgrade the level of M DA, raise the autophagy level in HUVECs,and decrease the expres-sion of iNOS and p38 MAPK phosphorylated protein in HUVECs.Conclusions LNT can improve HG-in- duced HUVEC injury,and the mechanism is related to regulating ROS/p38 MAPK pathway to enhance auto¬phagy levels and improve intracellular oxidative stress.
