The regulating effect of Collybistin on Neuropathic Pain
- Author:
Jia WEI
1
;
Shao-Li DING
1
;
Xue -Ming GAO
1
;
Ya-Min ZHANG
1
;
Yan-Qing SUN
1
;
Jia WEI
2
;
Yan-Qing SUN
2
;
Shu-Jin WU
Author Information
- Publication Type:Journal Article
- Keywords: collybistin; pain sensitization; peripheral nerve injury; postsynaptic potential; spinal cord; synaptic transmission
- From: Chinese Pharmacological Bulletin 2022;38(11):1711-1716
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the role of collybistin in the regulation of pain transmission. Methods The distribution of collybistin in spinal cord was observed by immunohistochemical staining. The role of collybis¬tin in pain transmission was evaluated by behavioral experiments. The effect of collybistin on inhibitory synap¬tic transmission was studied by electrophysiological ex¬periments. Results Collybistin was distributed in spi¬nal cord neurons; ShRNA-collybistin induced pain sen-sitization of intact mice ( P < 0. 05 ) . Overexpression of collybistin in spinal cord significantly alleviated pain sensitization induced by peripheral nerve injury ( P <0. 05 ) . ShRNA-collybistin also significantly reduced the amplitudes and frequencies of miniature inhibitory postsynaptic currents (mlPSCs) in superficial neurons of spinal cord dorsal horn (P <0. 05) . Overexpression of collybistin in spinal cord could reverse the effects of peripheral nerve injury on mlPSCs (P <0. 05). Con¬clusions Collybistin is involved in pain sensitization induced by peripheral nerve injury in mice.
