Effects of lentinan on dendritic cell metabolism
- Author:
Li LIU
1
;
Bao-Chen ZHANG
1
;
Xiu-Yun LI
1
;
En-Hui WU
1
;
Zhong-Feng SHI
1
;
Xiang-Liang DENG
1
;
Xiang-Liang DENG
2
Author Information
- Publication Type:Journal Article
- Keywords: arginine metabolism; dendritic cells; differential metabolites; lentinan; metabolomics; urea cycle
- From: Chinese Pharmacological Bulletin 2023;39(5):932-938
- CountryChina
- Language:Chinese
- Abstract: Aim To study the effects of lentinan(LNT)on the metabolism of dendritic cells(DCs)by metabonomics, and uncover the potential mechanism of its regulation of DC function. Methods DC2.4 cells were co-incubated with LNT for 24 h, and the activity of the cells was detected by thiazolyl blue tetrazolium bromide(MTT)assay. The contents of interleukin-6(IL-6), tumor necrosis factorα(TNF-α)and interleukin-12(IL-12)in supernatant were detected by enzyme-linked immunosorbent assay(ELISA). The metabolic general changes of DC2.4 cells were detected by Ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-QTOF/MS), and the differential metabolites were analyzed by multi-distance covariates and bioinformatics, partial least squares-discriminant analysis(PLS-DA). Finally, metabolic pathway analysis was performed by MetaboAnalyst 5.0. Results LNT did not significantly inhibit the activity of DC2.4 cells at the dose of 25100 mg·L-1. LNT(100 mg·L-1)could significantly stimulate the secretion of IL-6, TNF-α and IL-12 in DC2.4 cells. 20 differential metabolites were identified in DC2.4 cells after being stimulated by LNT(100 mg·L-1), which involved 25 metabolic pathways including urea cycle, arginine and proline metabolism. Conclusion The regulation of LNT on DC function involves a variety of amino acid metabolism.
