Casticin inhibits migration and invasion of hepatocellular carcinoma MHCC97H cells through miR-148a-3p/Wnt1 signaling pathway
- Author:
Lei LOU
1
;
Liang-Hou WANG
1
;
Zhi WANG
1
;
Xiang LI
1
Author Information
- Publication Type:Journal Article
- Keywords: casticin; cell invasion; cell migration; hepatocellular carcinoma; MHCC97H cells; miR-148a-3p/Wnt1 signaling pathway
- From: Chinese Pharmacological Bulletin 2023;39(5):868-875
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the effect of casticin (CAS) on the migration and invasion of MHCC97H cells and preliminarily explore the molecular mechanism. Methods CCK-8 kit was used to detect the effect of different concentrations of CAS on the viability of MHCC97H cells; cell migration and invasion assays were carried out in groups to assess the migration and invasion ability of MHCC97H cells; reverse transcription fluorescence quantitative PCR (RT-qPCR) was performed to detect the miR-148a-3p and Wnt1 mRNA expression in MHCC97H cells after CAS treatment; migration-invasion related proteins (MMP2, MMP9) and Wnt1 protein expression were detected by Western blot; Dual-Luciferase reporter gene was used to detect the binding of miR-148a-3p to Wnt1 3′-UTR. Results CAS significantly inhibited the viability of MHCC97H cells. The IC
