Effects of salidroside on proliferation, migration, invasion and apoptosis of 97H cells

Bing JIANG; Tao YANG; Long-Fei FENG; Hai-Xiang SU; Bing JIANG; Tao WANG; Hai-Xiang SU

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Effects of salidroside on proliferation, migration, invasion and apoptosis of 97H cells

Author: Bing JIANG ¹ ; Tao YANG ¹ ; Long-Fei FENG ¹ ; Hai-Xiang SU ¹ ; Bing JIANG ² ; Tao WANG ² ; Hai-Xiang SU ²
Author Information

1. School of Basic Medicine, Gansu University of Traditional Chinese Medicine
2. Gansu Provincial Academic Institute for Medical Research, Gansu Provincial Cancer Hospital
Publication Type:Journal Article
Keywords: apoptosis; invasion; liver cancer; migration; proliferation; salidroside
From: Chinese Pharmacological Bulletin 2023;39(3):445-452
CountryChina
Language:Chinese
Abstract: Aim To study the effect of salidroside on the proliferation, migration, invasion and apoptosis of human highly metastatic liver cancer cells ( 97 H cells) . Methods Multifunctional cell analyzer was used to determine the effect of salidroside on the proliferation of 97H cells. Scratch assay was used to determine the effect of salidroside on the migration ability of 97H cells. Transwell assay was used to determine the effect of salidroside on the invasion ability of 97 H cells. Inverted microscope was used to observe the effect of salidroside on the morphology of 97H cells. Transmission electron microscope was employed to observe the effect of salidroside on mitochondria in 97 H cells. Flow cytometry was used to analyze the effect of salidroside on apoptosis and cycle distribution of 97 H cells. q-PCR was used to determine the effect of salidroside on related apoptosis genes in 97H cells.Western blot was used to determine the effect of salidroside on related migration, invasion and apoptosis proteins in 97H cells. Results Compared to blank group, salidroside treatment inhibited the proliferation, migration and invasion of 97H cells, and induced apoptosis of 97H cells. Salidroside could up-regulate the relative expression of Caspase-3 (P <0.05). Salidroside could up-regulate the levels of E-cad, Bax, Caspase-3 and Caspase-9 proteins (P <0.05), and down-regulate the levels of N-cad, Girdin and Bcl-2 proteins (P < 0. 05 ). Conclusions Salidroside has inhibitory effects on the proliferation, migration and invasion of 97H cells, and induces apoptosis of 97H cells through mitochondrial pathway.