Effect of FATP5 gene silencing on fatty hepatic cell inflammation
- Author:
Tian-Qi WANG
1
;
Wen-Long SUN
2
;
Fu-Yuan YANG
3
;
Jing-Da LI
4
Author Information
- Publication Type:Journal Article
- Keywords: FATP5 receptor; inflammation; NF-kB; nonalcoholic fatty liver; shRNA gene silence; TNF-a
- From: Chinese Pharmacological Bulletin 2023;39(4):692-699
- CountryChina
- Language:Chinese
- Abstract: Aim To determine the effect of FATP5 gene silencing on fatty hepatic cell inflammation and to explore its possible mechanism. Methods Five shR-NA sequences were designed and synthesized. The efficient FATP5-shRNA was screened by the siCHECK™ system. After preparing the FATP5-shRNA lentivirus, the FATP5 gene silence hepatic cell lines was obtained by HepG2 cell infection and puromycin screening. The FATP5 silencing efficiency was detected by Western blot. Then the oleic acid induced ROS and MDA generation, TNF-a and IL-6 protein expression and secretion, and NF-kB activation in FATP5 gene silence cells were analyzed by the detection kit, Western blot, nucleo-plasmic separation and reporter gene system. Results In the gene silence cells, FATP5 protein expression was reduced by 90% and the lipid accumulation was also significantly inhibited. Moreover, the FATP5 knockdown could reduce the oleic acid induced ROS and MDA generation, and suppress the NF-kB activation, thereby inhibiting the protein expression and secretion of TNF-a and IL-6. Conclusions FATP5 gene silence inhibits fatty hepatic cell inflammation, and its mechanism may be related to the inhibition of oxidative stress and lipid peroxidation.
