Early changes in retinal structure and BMP2 expression in the retina and crystalline lens of streptozotocin-induced diabetic pigs.
10.5625/lar.2017.33.3.216
- Author:
Jae Seung JEONG
1
;
Woon Kyu LEE
;
Yeon Sung MOON
;
Na Rae KIM
Author Information
1. Department of Ophthalmology and Inha Vision Science Laboratory, Inha University School of Medicine, Incheon, Korea. drmys@inha.ac.kr, nrkim@inha.ac.kr
- Publication Type:Original Article
- Keywords:
BMP2;
diabetes mellitus;
lens;
pigs;
retina;
streptozotocin
- MeSH:
Blotting, Western;
Crystallins*;
Diabetes Mellitus;
Eosine Yellowish-(YS);
Fasting;
Ganglion Cysts;
Glucose;
Gyeonggi-do;
Hematoxylin;
Injections, Intravenous;
Lens, Crystalline*;
Niacinamide;
Retina*;
Retinaldehyde*;
Streptozocin;
Swine*
- From:Laboratory Animal Research
2017;33(3):216-222
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: This study aims to evaluate early changes in retinal structure and BMP2 expression in the retina and crystalline lens by comparing streptozotocin-induced diabetic pigs and normal control group pigs. METHODS: Five eye samples from five diabetic Micro-pigs (Medikinetics, Pyeongtaek, Korea) and five eye samples from five control pigs bred in a specific pathogen-free area were used. Diabetes was developed through intravenous injection of nicotinamide and streptozotocin, and the average fasting glucose level was maintained at 250 mg/dL or higher for 16 weeks. To evaluate BMP2 expression in the retina and crystalline lens, Western blotting was performed. RESULTS: In Hematoxylin and Eosin staining, most diabetic pigs showed structural abnormalities in the inner plexiform layer. The number of nuclei in the ganglion cell layer within the range of 10⁴µm² was 3.78±0.60 for diabetic pigs and 5.57±1.07 for control group pigs, showing a statistically significant difference. In immunohistochemical staining, diabetic retinas showed an overall increase in BMP2 expression. In Western blotting, the average BMP2/actin level of diabetic retinas was 1.19±0.05, showing a significant increase compared to the 1.06±0.03 of the control group retinas (P=0.016). The BMP2/actin level of diabetic crystalline lenses was similar to the control group crystalline lenses (P=0.730). CONCLUSIONS: Compared to control group pigs, the number of nuclei in the inner nuclear layer of retinas from streptozotocin-induced diabetic pigs decreased, while an increase in BMP2 expression was observed in the retina of diabetic pigs.
