Study of 3-bromopyruvate on regulating imbalance of apoptosis/autophagy in fibroblast-like synoviocytes through AMPK/mTOR pathway
- Author:
Ya-Ting WANG
1
;
Hao XIAN
1
;
Xiu-Rong BAO
1
;
Han-Meng ZHANG
1
;
Yi-Ning SONG
1
;
Fang WEI
1
;
Ying-Mei WEI
1
;
Ying WANG
1
Author Information
- Publication Type:Journal Article
- Keywords: 3-bromopyruvate; AMPK/mTOR signaling pathway; apoptosis; autophagy; fibroblast-like synoviocytes; rheumatoid arthritis
- From: Chinese Pharmacological Bulletin 2023;39(8):1463-1469
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the regulatory effects of 3-bromopyruvate (3-BrPA) on apoptosis and autophagy of fibroblast-like synoviocytes (FLS) in rats based on AMPK/mTOR signaling pathway and the underlying mechanism. Methods FLS of rats in vitro were cultured and induced by tumor necrosis factor-α (TNF-α) to construct a model of rheumatoid arthritis (R A). MTT assay was used to explore the optimal concentration of TNF-α and 3 -BrPA for induction and treatment of FLS. The effects of 3-BrPA on the migration and invasion of FLS were detected by Wound healing assay and Transwell assay. The apoptosis of FLS was tested by flow cytometry and mitochondrial membrane potential assay kit (JC-1). Moreover, FLS autophagic flux was detected by mCherry-EGFP-LC3B-overexpressed plasmids, and the expression of apoptosis/autophagy-related proteins as well as AMPK/mTOR pathway-related proteins were detected by Western blot. Results 3-BrPA (15 μmol • L) significantly inhibited the proliferation, migration, and invasion of FLS stimulated by TNF-a (25 μg • L
