The molecular mechanism of Qizhu anti-cancer prescription in treatment of primary liver cancer based on network pharmacology and experimental verification
- Author:
Meng-Qing MA
1
;
Jia-Ling SUN
1
;
Rui HU
1
;
Wen-Xing FENG
1
;
Zhi-Yi HAN
1
;
Xin-Feng SUN
1
;
Wen-Feng MA
1
;
Wei ZHANG
1
;
Jian-Ping CHEN
1
;
Xiao-Zhou ZHOU
1
;
Meng-Qing MA
2
;
Jia-Ling SUN
2
;
Rui HU
2
;
Wen-Xing FENG
2
;
Zhi-Yi HAN
2
;
Xin-Feng SUN
2
;
Wen-Feng MA
2
;
Wei ZHANG
2
;
Xiao-Zhou ZHOU
2
;
Rui HU
3
;
Jian-Ping CHEN
4
Author Information
- Publication Type:Journal Article
- Keywords: mechanism; molecular docking; network pharmacology; primary liver cancer; Qizhu anti-cancer prescription; signaling pathway
- From: Chinese Pharmacological Bulletin 2023;39(12):2369-2377
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the mechanism of Qizhu anti-cancer prescription ( QZACP) inthe treatment of primary liver cancer using network pharmacology and molecular docking. Methods Drugs and primary liver cancer ( PLC) -related targets were found according to TCMSP database and disease databases such as GeneCard, the key chemical components and core targets were screened by Cytoscape 3. 9. 1 and String platform respectively, and a network relationship diagram of traditional Chinese medicine-active component-target was constructed by using Cytoscape 3.9. 1. GO functional analysis and KEGG pathway analysis were performed using DAVID platform, visualized by R 4. 1. 1 software, and finally the core clustered proteins were analyzed by CytoNCA plug-in to obtain the core action targets, and the core components and key targets were verified by using molecular docking technology and the pharmacodynamic mechanism of QZACP was further verified by animal experiments. Results The active ingredients of QZACP in the treatment of primary liver cancer may be quercetin, glycyrrhizin, Denudatin B, isoflavanone, sanguinarol, etc. ; the potential targets were STAT3, EGFR, AKT1 etc. ; the related pathways were mainly PI3K-Akt signaling pathway,MAPK signaling pathway,etc. ; molecular docking showed that the core compounds had better integrating conformation with the key targets. In addition, QZACP could inhibit the growth of tumor in nude mice and decrease the expression of STAT3, EGFR and AKT1. Conclusions Qizhu anti-cancer prescription may have some positive significance in the treatment of primary liver cancer, which may be related to the regulation of PI3K/Akt signaling pathway.
