Analysis of Chemical Components and Tissue Distribution of Lujiao Formula Based on UPLC-Q-Orbitrap-MS
10.13422/j.cnki.syfjx.20240168
- VernacularTitle:基于UPLC-Q-Orbitrap-MS的鹿角方化学成分及组织分布分析
- Author:
Jianwei ZHANG
1
;
Wei LIU
1
;
Qin SHEN
1
;
Li LIU
1
Author Information
1. Third Grade Laboratory of Traditional Chinese Medicine（TCM） Preparations， National Administration of TCM，Shuguang Hospital Affiliated to Shanghai University of TCM， Shanghai 201203，China
- Publication Type:Journal Article
- Keywords:
Lujiao formula;
Epimedii Folium;
Ligustri Lucidi Fructus;
Psoraleae Fructus;
Corni Fructus;
Citri Reticulatae Pericarpium;
chemical composition;
tissue distribution;
ultra performance liquid chromatography-quadrupole-electrostatic field orbitrap high resolution mass spectrometry（UPLC-Q-Orbitrap-MS）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2024;30(8):148-156
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo qualitatively analyze the chemical constituents and their tissue distribution in Lujiao formula based on ultra performance liquid chromatography-quadrupole-electrostatic field orbitrap high resolution mass spectrometry（UPLC-Q-Orbitrap-MS）. MethodThe separation was performed on an ACQUITY UPLC® BEH C18 column（2.1 mm×100 mm， 1.7 μm） with the mobile phase of 0.1% formic acid aqueous solution（A）-methanol（B） in a gradient elution（0-2 min， 4%B； 2-6 min 4%-12%B； 6-38 min， 12%-70%B； 38-38.5 min， 70%B； 38.5-39 min， 70%-95%B； 39-43 min， 95%B； 43-43.1 min， 95%-4%B； 43.1-45 min， 4%B）， the flow rate was 0.3 mL·min-1 with the column temperature of 40 ℃ and the injection volume of 5 µL. The data were acquired by an electrospray ionization（ESI） in the full scanning mode of positive and negative ions， the scanning rang was set at m/z 100-1 500， the collision energies were 10， 20， 40 eV. Retention time， precise relative molecular mass and secondary mass spectrometry fragment ions were used to identify the compounds in Lujiao formula and analyze their tissue distribution， combing with self-established database and comparing with standard substances and published literature data. ResultA total of 260 compounds， including 156 flavonoids， 43 terpenoids， 18 coumarins， 13 organic acids， 7 phenylethanoids， 7 alkaloids and 16 others， were identified or hypothesized from Lujiao formula， 68 of which were identified by comparison with standard substances. And the results of tissue distribution showed that 100， 143， 129 and 126 prototype components were detected in blood， heart， liver and kidney， respectively. ConclusionThe chemical composition of Lujiao formula and their tissue distribution were systematic analyzed， which can provide reference for the quality control， clinical application， pharmacokinetics and pharmacodynamic material basis of Lujiao formula and its medicinal materials.
