The effect of platelet infusion on the repair of bone marrow hematopoietic niche damage in mice induced by (60)Co radiation and hematopoietic reconstruction after bone marrow transplantation.
10.3760/cma.j.issn.0253-2727.2023.08.004
- VernacularTitle:血小板输注对
60Co照射所致小鼠骨髓造血龛损伤修复和骨髓移植后造血重建的影响
- Author:
Yun LIU
1
;
Shuang DING
1
;
Jing Fang SUN
1
;
Peng Peng LI
1
;
Xiao Qian LI
2
;
Ling Yu ZENG
2
;
Kai Lin XU
2
;
Jian Lin QIAO
2
Author Information
1. Department of Clinical Laboratory, the Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China.
2. Blood Disease Institute, Xuzhou Medical University, Key Laboratory of Bone Marrow Stem Cell, Department of Hematology, the Affiliated Hospital of Xuzhou Medical University, Xuzhou 221000, China.
- Publication Type:Journal Article
- Keywords:
Bone marrow transplantation;
Hematopoietic microenvironment;
Mouse;
Platelet transfusion
- MeSH:
Mice;
Animals;
Bone Marrow Transplantation;
Bone Marrow;
Mice, Inbred C57BL;
Hematopoietic Stem Cells;
Bone Marrow Diseases;
Hematopoietic Stem Cell Transplantation;
Mice, Inbred BALB C
- From:
Chinese Journal of Hematology
2023;44(8):635-641
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the effect of platelets on hematopoietic stem cell (HSCs) implantation in mice with radiation-induced bone marrow injury and bone marrow transplantation models. Methods: ①Male C57BL/6 mice were divided into a single irradiation group and a radiation infusion group after receiving (60)Co semimyeloablative irradiation for 18-10 weeks. The irradiation infusion group received 1×10(8) platelets expressing GFP fluorescent protein. ② The allogeneic bone marrow transplantation model was established. The experimental groups included the simple transplantation group (BMT) and the transplantation infusion group (BMT+PLT). The BMT group was infused through the tail vein only 5 × 10(6) bone marrow cells, the BMT+PLT group needs to be infused with bone marrow cells at the same time 1× 10(8) platelets. ③ Test indicators included peripheral blood cell and bone marrow cell counts, flow cytometry to detect the proportion of hematopoietic stem cell (HSC) and hematopoietic progenitor cells, bone marrow cell proliferation and apoptosis, and pathological observation of vascular niche damage and repair. Results: ①On the 3rd, 7th, 14(th), and 21st days after irradiation, the bone marrow cell count of the infusion group was higher than that in the single irradiation group (P<0.05), and the peripheral blood cell count was also higher. A statistically significant difference was found between the white blood cell count on the 21st day and the platelet count on the 7th day (P<0.05). In the observation cycle, the percentage of bone marrow cell proliferation in the infusion group was higher, while the percentage of apoptosis was lower. ② The results of bone tissue immunofluorescence after irradiation showed that the continuity of hematopoietic niche with red fluorescence was better in the irradiation infusion group. ③The chimerism percentage in the BMT+PLT group was always higher than that in the BMT group after transplantation.④ The BMT+PLT group had higher bone marrow cell count and percentage of bone marrow cell proliferation on the 7th and 28th day after transplantation than that in the BMT group, and the percentage of bone marrow cell apoptosis on the 14th day was lower than that in the BMT group (P<0.05). After the 14th day, the percentage of stem progenitor cells in the bone marrow cells of mice was higher than that in the BMT group (P<0.05). ⑤The immunohistochemical results of bone marrow tissue showed that the continuity of vascular endothelium in the BMT+PLT group was better than that in the BMT group. Conclusion: Platelet transfusion can alleviate the injury of vascular niche, promotes HSC homing, and is beneficial to hematopoietic reconstruction.
