Protective effect of ginsenoside Rg1 on hypoxic-ischemic brain damage and neuronal cell apoptosis in young rats

Mingxiao ZHANG; Feng FANG; Yan YANG; Junli FAN; Peng YUAN

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Protective effect of ginsenoside Rg1 on hypoxic-ischemic brain damage and neuronal cell apoptosis in young rats

VernacularTitle:人参皂苷Rg1对幼龄大鼠缺氧缺血性脑损伤和神经元凋亡的保护作用
Author: Mingxiao ZHANG ¹ ; Feng FANG ¹ ; Yan YANG ¹ ; Junli FAN ² ; Peng YUAN ³
Author Information

1. Anatomy Department，Medical College of Luoyang Polytechnic, Luoyang 471000
2. Department of Oncology, The First Affiliated Hospital of Henan University of Science and Technology, Luoyang 471000
3. Department of Anatomy, Tianjin Medical College, Tianjin 300000, China
Publication Type:Journal Article
Keywords: ginsenoside Rg1; hypoxic-ischemic brain damage; neuronal cell
From: Journal of Xi'an Jiaotong University(Medical Sciences) 2021;42(5):693-699
CountryChina
Language:Chinese
Abstract: 【Objective】 To explore the effects of ginsenoside Rg1 （ginsenoside Rg1） on cell apoptosis， oxidative stress and inflammation in young rats with hypoxic-ischemic brain damage （HIBD）. 【Methods】 Young Wistar rats were divided into healthy control group， model group （HIBD）， and HIBD+ginsenoside Rg1 group. Jumping test was used to detect the number of mistakes in each group of rats， brain tissue wet and dry weight method was used to calculate the brain water content and brain index of each group of rats. Hematoxylin-eosin （HE） staining was used to observe the degree of brain damage； TUNEL staining to observe the apoptosis level of brain tissue； Nissl body staining to detect neuronal cell apoptosis； Western blot to detect brain tissue Bax/Bcl-2， caspase-3， caspase-9 protein expressions； malondialdehyde （MDA） and superoxide dismutase （SOD） activity detection kit to detect MDA and SOD content， respectively； and ELISA to detect peripheral blood IL-6， iNOS， IL-4 contents. 【Results】 Compared with the model group， 20 mg/kg and 40 mg/kg ginsenoside Rg1 significantly reduced the number of mistakes in the jumping-off test of HIBD young rats， and brain index brain water rate and neuronal cell apoptosis （P<0.05）； alleviated brain tissue damage； down-regulated the protein expression levels of Bax/Bcl-2， cleaved cas9/cas9 and cleaved cas3/cas3 （P<0.05）； reduced IL-6 and iNOS protein content； and increased IL-4 protein content （P<0.05）. 【Conclusion】 Ginsenoside Rg1 improves brain tissue damage in young HIBD mice and inhibits neuronal cell apoptosis.