Magnesium citrate inhibits hyperphosphorus-induced VSMCs calcification through activating calcium-sensitive receptor
- VernacularTitle:枸橼酸镁激活钙敏感受体抑制高磷诱导的血管平滑肌细胞钙化
- Author:
Zhihui YAO
1
;
Tuo HAN
1
;
Yajie FAN
1
;
Hong GONG
1
;
Lixia WANG
1
;
Yiwen WANG
1
;
Congxia WANG
1
Author Information
- Publication Type:Journal Article
- Keywords: magnesium citrate; VSMCs calcification; calcium sensitive receptor
- From: Journal of Xi'an Jiaotong University(Medical Sciences) 2022;43(3):331-336
- CountryChina
- Language:Chinese
- Abstract: 【Objective】 To investigate the inhibitory effect of magnesium citrate （MgCit） on hyperphosphorus-induced VSMCs calcification and its mechanisms. 【Methods】 VSMCs were divided into the following groups： normal control group， high-phosphorus group， low-dose MgCit group， high-dose MgCit group and high-dose MgCit+NPS2143 （calcium-sensitive receptor inhibitor） group. Alizarin red staining was used for semi-quantitative analysis of VSMCs calcification and the calcium content in VSMCs was detected by the commercial kit. The osteogenic transdifferentiation parameters including alkaline phosphatase （ALP） activity， as well as mRNA and protein levels of smooth muscle 22α （SM22α）， runt-related transcription factor 2 （RUNX2） and bone morphogenetic protein 2 （BMP2）， were detected in each group. 【Results】 Compared with those in the control group， the calcium content of VSMCs in the model group was increased， ALP activity， and the mRNA and protein expressions of RUNX2 and BMP2 were increased， and the mRNA expression of SM22α was decreased （P<0.05）. MgCit could reduce VSMCs calcification， decrease ALP activity， decrease mRNA and protein levels of RUNX2 and BMP2， and increase mRNA levels of SM22α induced by high-phosphorous （P<0.05）. The above effects of MgCit were reduced when NPS2143 was administered simultaneously （P<0.05）. 【Conclusion】 MgCit can reduce VSMCs calcification and osteogenic transdifferentiation induced by high phosphorus through activating of CaSR.
