Effect of LINC00174 on the Malignant Proliferation of Multiple Myeloma Cells and Its Related Mechanism.
10.19746/j.cnki.issn.1009-2137.2023.04.024
- Author:
Jing-Jing WANG
1
;
Cui-Ping ZHAO
1
;
Shi-Guang WANG
2
,
3
Author Information
1. Department of Internal Medicine, Zhengzhou University of Industry Technology, Zhengzhou 451100, Henan Province, China.
2. Department of Internal Medicine, Zhengzhou University of Industry Technology, Zhengzhou 451100, Henan Province, China.E-mail: wsgsxy@
3. com.
- Publication Type:Journal Article
- Keywords:
FOXP1;
LINC00174;
miR-150;
multiple myeloma;
proliferation
- MeSH:
Humans;
Apoptosis;
Caspase 3;
Cell Line, Tumor;
Cell Proliferation;
Forkhead Transcription Factors;
Ki-67 Antigen;
MicroRNAs/genetics*;
Multiple Myeloma/pathology*;
Repressor Proteins;
RNA, Small Interfering;
RNA, Long Noncoding/genetics*
- From:
Journal of Experimental Hematology
2023;31(4):1085-1092
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the biological function of LINC00174 in multiple myeloma (MM).
METHODS:Real-time quantitative polymerase chain reaction (RT-qPCR) was used to detect the expressions of LINC00174 and miR-150 in peripheral blood of MM patients and MM cell lines. EdU staining and flow cytometry were used to detect the effects of LINC00174 and miR-150 on the proliferation and apoptosis of MM cells. Western blot was used to detect the expressions of proliferation marker nuclear-related antigen Ki67, apoptosis-related protein cleaved caspase-3 and transcription factor forkhead box protein P1 (FOXP1). Bioinformatics and dual-luciferase reporter assay were used to verify the targeting relationship between LINC00174 and miR-150 and the targeting relationship between miR-150 and FOXP1.
RESULTS:The level of LINC00174 was significantly increased in peripheral blood of MM patients and MM cell lines (P <0.05). Compared with NC-siRNA group, the expression of LINC00174 was significantly reduced in LINC00174-siRNA group, the proliferation of U266 cells was reduced, the apoptosis rate was significantly increased, the level of Ki67 protein was reduced, and the level of cleaved caspase-3 protein was increased (all P <0.05). LINC00174 targeted regulation of the expression of miR-150. Compared with LINC00174-siRNA+NC inhibitor group, the expression of miR-150 in U266 cells in LINC00174-siRNA+miR-150 inhibitor group was significantly reduced, the cell proliferation was enhanced, the apoptosis rate was reduced, the level of Ki67 protein was increased, and the level of cleaved caspase-3 was decreased (all P <0.05). FOXP1 is the target gene of miR-150. Compared with NC mimic group, the expression of FOXP1 protein in miR-150 mimic group was significantly reduced, the cell proliferation was reduced, the apoptosis rate was significantly increased, Ki67 protein level was decreased, and the level of cleaved caspase-3 was increased. Compared with miR-150 mimic + vector group, the expression of FOXP1 protein in miR-150 mimic + pcDNA-FOXP1 group was significantly increased, the cell proliferation was enhanced, the apoptosis rate was reduced, the level of Ki67 protein was increased, and the level of cleaved caspase-3 was decreased (all P <0.05).
CONCLUSION:LINC00174 promotes the proliferation of MM cells and inhibits cell apoptosis by regulating the miR-150/ FOXP1 axis.
