Comparative evaluation of indirect immunofluorescent antibody test with enzyme-linked immunosorbent assay in serodiagnosis of human neurocysticercosis.
- Author:
Kee Seon EOM
1
;
Seung Yull CHO
;
Han Jong RIM
Author Information
- Publication Type:Original Article
- MeSH: parasitology-helminth-cestoda; Taenia solium; cysticercus; enzyme-linked immunosorbent assay; cerebrospinal fluid; brain; IgG; immunofluorescence; immunology
- From:The Korean Journal of Parasitology 1988;26(1):27-32
- CountryRepublic of Korea
- Language:English
- Abstract: The applicability of indirect immunofluorescent antibody test (IFAT) was compared with enzyme-linked immunosorbent assay (ELISA) in sera from 163 cases of confirmed neurocysticercosis, 101 other neurologic and parasitic diseases and 100 normal controls. As antigen, frozen sections of a Taenia solium metacestode from a human brain was used in IFAT and cystic fluid was used in ELISA. For the detection of specific IgG antibody, IFAT was equally sensitive (89. 6%) and specific (85. l%) as ELISA. The antibody titers by IFAT were correspondingly increased with mean absorbance of ELISA. The corresponding rate of positivity in the two techniques was 90.8%. Except for the difficulty in detecting antibodies in cerebrospinal fluid (CSF), IFAT was concluded to be very useful for the serodiagnosis of human neurocysticercosis.
