Identification of chemical constituents in Guizhi Fuling Capsules by UPLC-Q-TOF-MS/MS.
10.19540/j.cnki.cjcmm.20191002.309
- Author:
Hui-Min YANG
1
;
Biao YANG
2
;
Yu-Mei HU
2
;
Liang CAO
2
;
Zhen-Zhong WANG
2
;
Ke-Jin ZHU
2
;
Wei XIAO
2
Author Information
1. China Pharmaceutical University Nanjing 211198, China Jiangsu Kanion Pharmaceutical Co., Ltd. Lianyungang 222001, China State Key Laboratory of New Techology for Chinese Medicine Pharmaceutical Process Lianyungang 222001, China.
2. Jiangsu Kanion Pharmaceutical Co., Ltd. Lianyungang 222001, China State Key Laboratory of New Techology for Chinese Medicine Pharmaceutical Process Lianyungang 222001, China.
- Publication Type:Journal Article
- Keywords:
Guizhi Fuling Capsules;
UPLC-Q-TOF-MS/MS;
chemical profile
- MeSH:
Capsules;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal/chemistry*;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2020;45(4):861-877
- CountryChina
- Language:Chinese
-
Abstract:
To qualitatively characterize the chemical composition of Guizhi Fuling Capsules using UPLC-ESI-Q-TOF-MS/MS. The analysis was performed on Agilent ZORBAX RRHD Eclipes Plus C_(18)(2.1 mm×100 mm, 1.8 μm) column,that was eluted with mobile phase consisting of acetonitrile and 0.1% formic acid in a gradient mode. The flow rate was 0.4 mL·min~(-1), and column temperature was 30 ℃. Tandem mass spectrometry was acquired in both negative and positive ESI modes. These components were further analyzed based on high-resolution mass-to-charge ratios, fragment ion species, reference substances and literature data. In conclusion, a total of 200 compounds were identified, in which 40 were verified with reference substances. The current study laid a foundation for in-depth studies of its mass balance and pharmacodynamics.
