Chinese expert consensus on clinical practice of quantitative real-time polymerase chain reaction techniques in molecular pathological diagnostics of tumor(2023 edition).
10.3760/cma.j.cn112152-20221231-00868
- VernacularTitle:基于实时荧光定量PCR技术的肿瘤分子病理检测临床实践中国专家共识(2023版)
- Collective Name:Society of Tumor Pathology of Chinese Anti-Cancer Association,;Society of Gene Diagnosis of Tumor of Chinese Anti-Cancer Association,;Society of Ultrastructural and Molecular Pathology of Chinese Research Hospital Association,;Beijing Quality Control and Quality Improvement Center of Pathology
- Publication Type:Journal Article
- Keywords:
Expert consensus;
Molecular pathology;
Molecular testing;
Quantitative real-time PCR;
Tumor
- MeSH:
Humans;
Real-Time Polymerase Chain Reaction;
Consensus;
East Asian People;
Neoplasms;
China
- From:
Chinese Journal of Oncology
2023;45(9):763-772
- CountryChina
- Language:Chinese
-
Abstract:
Quantitative real-time polymerase chain reaction (qPCR) is one of the most widely used molecular pathological diagnostic techniques in China due to its advantages of the simple operation, short turnaround time, high sensitivity, and standardizable result analysis. However, in clinical practice, there is not yet an expert consensus to guide molecular pathological diagnostics of tumor using qPCR techniques in terms of validation and verification of method performance, quality management and interpretation of complex results. Therefore, this expert consensus aims to provide standardized opinions on the practical application of qPCR techniques, and suggestions on how to deal with common problems and abnormal results, and reach a Chinese expert consensus on the clinical practice of qPCR techniques in molecular pathological diagnostics of tumor, in order to standardize the testing process, improve the accuracy of results, and promote the clinical applications of qPCR techniques.
