Dihydroartemisinin Suppresses Proliferation of Oral Squamous Cell Carcinoma Cells by Promoting Autophagy
10.3971/j.issn.1000-8578.2024.23.0641
- VernacularTitle:双氢青蒿素通过诱导自噬抑制口腔鳞状细胞癌细胞增殖
- Author:
Dan ZHANG
1
;
Hao ZHANG
;
Li HE
Author Information
1. Hubei University of Medicine, Shiyan 442000, China
- Publication Type:Research Article
- Keywords:
Dihydroartemisinin;
Oral squamous cell carcinoma;
Autophagy
- From:
Cancer Research on Prevention and Treatment
2024;51(1):22-26
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of dihydroartemisinin (DHC) on the proliferation capacity of human oral squamous carcinoma cells and its mechanism of action. Methods The viability and colony formation ability of CAL27 cells treated with different concentrations of dihydroartemisinin was measured by CCK-8 and colony formation assay. The expression of proteins related to proliferation and autophagy was determined by Western blot. Potential targets for DHA inhibition of the biological behavior of oral cancer were screened based on network pharmacology and bioinformatics. Measurement was conducted after the cells were cotreated with autophagy blocker 3-methyladenine and autophagy inducers rapamycin and dihydroartemisinin. Results Dihydroartemisinin significantly reduced the proliferation viability and clone formation ability of CAL27 cells in a concentration-dependent manner. The PCNA expression level also decreased substantially. DHA suppressed oral cancer targets involving autophagy-related pathways. DHA intervention increased the expression of intracellular autophagy-related proteins Beclin-1 and LC3. After co-treatment of DHA combined with autophagy blocker, the proliferation viability and clone formation ability of CAL27 cells decreased. The expression of PCNA increased, and the expression of Beclin-1 and LC3 decreased. Conclusion Dihydroartemisinin could inhibit the proliferative capacity of oral squamous carcinoma cells in vitro, and its effect may be correlated with the induction of autophagy.
