miR-139-5p targeting DNMT1 inhibits the growth and metastasis of glioblastoma

Chuankun LI; Wei WANG; Haitao JIANG; Yaling HE

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miR-139-5p targeting DNMT1 inhibits the growth and metastasis of glioblastoma

VernacularTitle:miR-139-5p靶向调控DNMT1抑制胶质母细胞瘤的生长和转移
Author: Chuankun LI ¹ ; Wei WANG ¹ ; Haitao JIANG ¹ ; Yaling HE ²
Author Information

1. Department of Neurosurgery, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710061
2. School of Energy and Power Engineering, Xi’an Jiaotong University, Xi’an 710049, China
Publication Type:Journal Article
Keywords: miR-139-5p; glioblastoma; DNA methyltransferase 1; neurofibromatosis type 2 (NF2) gene; cell proliferation; cell invasion
From: Journal of Xi'an Jiaotong University(Medical Sciences) 2021;42(2):238-244
CountryChina
Language:Chinese
Abstract: 【Objective】 To investigate the expression and biological role of miR-139-5p in glioblastoma and the regulatory effect of miR-139-5p on DNA methyltransferase 1 (DNMT1). 【Methods】 qRT-PCR was used to detect the expression of miR-139-5p in glioblastoma tumor tissue, paired paracancerous tissue, human normal glioma cell line HEB, and human glioma cell line U251. The expression of miR-139-5p in U251 cells was up-regulated by transfection of miR-139-5p mimetics, and the expression of DNMT1 was down-regulated by transfection of DNMT1-targeted siRNA (DNMT1-siRNA). The expression of DNMT1 and neurofibromatosis type 2 (NF2) in tissues and cells was detected by qRT-PCR, Western blotting, immunohistochemistry and immunofluorescence. The cell counting kit-8 (CCK-8), flow cytometry and Matrigel Transwell were used to evaluate the proliferation, apoptosis and invasion ability of U251 cells. 【Results】 Compared with paracancerous tissues or HEB cells, miR-139-5p expression in glioblastoma tissues and U251 cells was suppressed (P<0.05). Compared with control cells, transfection of miR-139-5p mimic significantly down-regulated the expression of DNMT1 and up-regulated the expression of NF2 (P<0.05). Compared with control cells, transfection of DNMT1-siRNA significantly promoted the expression of NF2 (P<0.05). Transfection of miR-139-5p mimetics or DNMT1-siRNA significantly induced U251 cell apoptosis and inhibited cell invasion (P<0.05). 【Conclusion】 miR-139-5p plays an anti-cancer role in glioblastoma, and it inhibits tumor proliferation and metastasis by targeting negative regulation of DNMT1.