Adiponectin alleviates LPS-induced inflammatory response of microglia through A20/NLRP3/caspase-1 pathway

Hui WANG; Qiang CHEN; Haibo LI; Zhanqin ZHANG

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Adiponectin alleviates LPS-induced inflammatory response of microglia through A20/NLRP3/caspase-1 pathway

VernacularTitle:脂联素通过A20/NLRP3/caspase-1途径抑制脂多糖诱导的小胶质细胞炎症反应
Author: Hui WANG ¹ ; Qiang CHEN ² ; Haibo LI ³ ; Zhanqin ZHANG ⁴
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1. Department of Pain, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710061, China
2. Department of Cardiovascular Surgery, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710061, China
3. Department of Cardiovascular Medicine, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710061, China
4. Department of Anesthesiology & Center for Brain Science, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710061, China
Publication Type:Journal Article
Keywords: adiponectin; microglia; tumor necrosis factor α-induced protein 3 (A20); NOD-, LRR- and pyrin domain-containing 3 (NLRP3); caspase-1
From: Journal of Xi'an Jiaotong University(Medical Sciences) 2021;42(4):529-533
CountryChina
Language:Chinese
Abstract: 【Objective】 To explore the mechanism of adiponectin (APN) on lipopolysaccharide (LPS)-induced microglial inflammatory response. 【Methods】 After APN intervention or/and LPS stimulation, microglia were harvested and divided into control group, APN group, LPS group, and LPS+APN group. The expressions and secretion of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in microglia were detected by q-PCR and ELISA assay, respectively. The expressions of tumor necrosis factor α-induced protein 3 (TNFAIP3/A20), NOD-, LRR- and pyrin domain-containing 3 (NLRP3) and caspase-1 were detected by Western blot. After silencing A20 expression in the microglia, the cells were treated with APN, and the expressions of A20, NLRP3 and caspase-1 were detected by Western blot again. 【Results】 The mRNA transcription levels (P=0.018, P=0.009) and secretion (P=0.000 1, P=0.000 1) of TNF-α and IL-1β in LPS-induced microglia were significantly decreased after APN intervention. Moreover, the expression of A20 was increased while the expressions of NLRP3 and caspase-1 declined in microglia of LPS+ APN group when compared with LPS group (P=0.001, P=0.003, P=0.006). However, the down-regulated expressions of NLRP3 and caspase-1 by APN were reversed by A20 silencing in microglia (P=0.012, P=0.024). 【Conclusion】 APN can inhibit NLRP3 and caspase-1 expressions in microglia by the up-regulated expression of A20 protein.