The regulation mechanism of HCBP6 mimic phosphorylation on triglyceride synthesis in hepatocytes
- VernacularTitle:HCBP6模拟磷酸化对肝细胞内三酰甘油合成的调控作用
- Author:
Xueliang YANG
1
;
Xiaojing LIU
2
;
Ming HAN
3
;
Shunai LIU
3
;
Jun CHENG
3
;
Shumei LIN
2
Author Information
- Publication Type:Journal Article
- Keywords: metabolism-associated fatty liver disease; triglyceride; HCBP6; phosphorylation; SREBP1c
- From: Journal of Xi'an Jiaotong University(Medical Sciences) 2021;42(4):502-507
- CountryChina
- Language:Chinese
- Abstract: 【Objective】 To investigate the regulation of HCBP6 mimic phosphorylation on triglyceride synthesis in hepatocytes so as to provide a molecular target for the treatment of metabolism-associated fatty liver disease. 【Methods】 We used site-directed mutagenesis to mimic constitutive phosphorylation and dephosphorylation of HCBP6 Ser-10 and Ser-151. Oil red O staining and triglyceride content determination were used to detect triglyceride levels in hepatocytes. The expressions of SREBP1c, ACC1 and FASN were detected by qRT-PCR and Western blotting. The Dual-Luciferase Report Gene System was used to detect SREBP1c promoter activity. 【Results】 HCBP6 Ser-10 phosphorylation promoted triglyceride synthesis. HCBP6 Ser-10 phosphorylation upregulated the expressions of SREBP1c, ACC1and FASN genes; HCBP6 Ser-10 phosphorylation enhanced the SREBP1c promoter activity. 【Conclusion】 HCBP6 Ser-10 phosphorylation can significantly enhance the activity of the SREBP1c promoter, upregulate the SREBP1c-FASN signal pathway transduction, and promote the synthesis of triglycerides.
