Evaluation of the performance of SPINK1/SPP1in diagnosis of hepatocellular carcinoma, alone or in combination
- VernacularTitle:血清SPINK1/SPP1联合诊断原发性肝癌的效能评价
- Author:
Jun LI
1
;
Zhao YANG
2
;
Xiaoning TONG
1
;
Jiaqi CUI
3
Author Information
- Publication Type:Journal Article
- Keywords: primary liver cancer; diagnostic model; SPINK1; SPP1; AFP
- From: Journal of Xi'an Jiaotong University(Medical Sciences) 2022;43(5):703-708
- CountryChina
- Language:Chinese
- Abstract: 【Objective】 To evaluate the performance of SPINK1/SPP1 in diagnosis of hepatocellular carcinoma (HCC) alone or in combination. 【Methods】 A total of 419 serum samples were collected and divided into four groups: normal control (n=93), chronic hepatitis B (CHB) (n=72), HBC related liver cirrhosis (LC) (n=77), and hepatocellular carcinoma (HCC) (n=177). Serum concentrations of SPINK1 and SPP1 were determined by ELISA kits. All parameters were first analyzed by significance tests among the groups. To distinguish tumors from non-tumors, a combination model was generated by multivariable binary Logistic regression using a comprehensive control group which consisted of the normal, the CHB and the LC groups. The performance of each indicator was judged by comparison of AUC, sensitivity, specificity and accuracy. 【Results】 The serum levels of SPINK1 and SPP1 were both significantly higher in HCC group than in all the others (P<0.000 1). ROC curve analysis showed that AFP, SPINK1 and SPP1 achieved AUC of 0.798 (0.755-0.845), 0.951 (0.931-0.971) and 0.939 (0.916-0.963), respectively. The combination model based on SPINK1 and SPP1 reached the highest AUC of 0.972 (0.957-0.986). AUC of AFP was significantly lower than that of the other indicators (P<0.000 1); meanwhile, AUC of SPINK1and SPP1, with no significant difference (P>0.05), was lower than that of the combination model (P=0.042 9 and P<0.001, respectively). With the cutoff of 14.28, AFP established sensitivity, specificity and accuracy of 0.678, 0.864, and 0.785, which were significantly lower than those in the other groups, with those of 0.949, 0.851 and 0.892 (SPINK1, cutoff=7.39); 0.915, 0.855 and 0.877 (SPP1, cutoff=8.14); 0.938, 0.918 and 0.926 (combination model, cutoff=0.403). AFP differed significantly from that in the other three groups (P<0.000 1). For comparison of SPINK1 or SPP1 with combination model, the differences were not statistically significant (both P>0.05). 【Conclusion】 The data identified SPINK1 and SPP1 as novel tumor biomarkers with greater robust efficiency than the currently used AFP for detection of hepatocellular carcinoma, alone or in combination.
