Animalexperiment on regulating effect of stored red blood cells transfusion on BMDMs in inflammatory conditions
10.13303/j.cjbt.issn.1004-549x.2023.08.005
- VernacularTitle:输注贮存红细胞在炎症条件下对巨噬细胞调节作用的动物实验
- Author:
Yanchun ZHANG
1
;
Jing JIN
1
;
Tao WU
1
Author Information
1. Department of Blood Transfusion Medicine, The Seventh Medical Center of PLA General Hospital, Beijing 100700, China
- Publication Type:Journal Article
- Keywords:
stored red blood cells(RBCs);
fresh red blood cells(RBCs);
red blood cells(RBCs) infusion;
inflammation;
bone marrow-derived macrophages(BMDMs);
liver tissue;
mice;
animal experiment
- From:
Chinese Journal of Blood Transfusion
2023;36(8):676-680
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To discuss the regulating effect of stored red blood cells (RBCs) transfusion on BMDMs in inflammatory conditions, and the relationship between stored RBCs transfusion and inflammatory response induced by bacterial infection. 【Methods】 Forty C57BL/6 male mice of 6-8 weeks (18-22 g/mouse) were randomly divided into experimental group and control group. Each mouse was infected with 200 µL Pseudomonas aeruginosa injecting into the tail vein, and 400 µL fresh (storage >14 d) and stored RBCs (storage <24 h) suspension was infused through the posterior venous plexus into the isoflurane anesthetized mice (1%-3%). The BMDMs of Pseudomonas aeruginosa-infected mice were cultured, and CD86, CD197, CD209 in BMDMs were detected using FCM (flow cytometry) technique. The livers were removed 2, 4 and 8 hours after RBCs infused, then F4/80, M1 and M2 in liver tissues were tested using qRT-PCR. The data were analyzed using statistical software 17.0. 【Results】 CD86 and CD197 (%) of experimental group and control group were 86 88±1.01 vs 79.24±2.65 and 38.59± 3.73 vs 25.95+0.86 (P<0.05), and CD209 (%) were 23.88±2.23 vs 21.91+3.58 (P>0.05). F4/80 of experimental group and control group 2, 4 and 8 hours after RBCs infused were 1.83±0.11 vs 0.75±0.06, 0.46±0.06 vs 0.33±0.06 (P<0.05) and 0.33± 0.03 vs 0.35±0.05 (P>0.05), respectively. iNOS, TNF-α, MCP1 of M1 in liver of experimental group and control group 2, 4 and 8 hours after RBCs infused were respectively: iNOS 3.44±0.20 vs 2.46±0.08, 9.25±0.55 vs 2.67±0.12, 2.80±0.08 vs 2.39 ±0.01; TNF-α 1.69±0.22 vs 1.13±0.03, 1.44±0.24 vs 0.96±0.09, 1.31±0.05 vs 0.96±0.06; MCP1 4.96±0.08 vs 4.28±0.27, 4.63±0.04 vs 2.07±0.09, 2.28±0.19 vs 1.33±0.03 (P<0.05). Arg1, Mrc2 and CD163 of M2 were respectively: Arg1 0.81±0.21 vs 0.82±0.18, 0.66±0.11 vs 0.58±0.09, 0.39±0.17 vs 0.37±0.15; Mrc2 0.99±0.91 vs 0.97±0.08, 0.98±0.12 vs 1.02±0.11, 0.59±0.19 vs 0.57±0.08; CD163 1.75±0.20 vs 1.69±0.18, 0.22±0.02 vs 0.21±0.01, 0.04±0.01 vs 0.03±0.01 (P>0.05). 【Conclusion】 Stored RBCs infusion can greatly promote the M1 polarization of BMDMs in liver.
