Preparation, physicochemical characterization and cytocompatibility study of self-assembled silk fibroin nanoparticles
10.16438/j.0513-4870.2023-1273
- VernacularTitle:丝素蛋白自组装纳米粒的制备、理化表征及细胞相容性研究
- Author:
Yu YAN
1
;
Jia-ling CHENG
2
;
Zi-han LIU
2
;
Hong-liang WANG
2
;
Yan-fang YANG
2
;
Jun YE
2
;
Wei-zhe JIANG
3
;
Yu-ling LIU
2
Author Information
1. College of Pharmacy, Guangxi Medical University, Nanning 530021, China; Beijing Key Laboratory of Drug Delivery Technology and Novel Formulation, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
2. Beijing Key Laboratory of Drug Delivery Technology and Novel Formulation, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
3. College of Pharmacy, Guangxi Medical University, Nanning 530021, China
- Publication Type:Research Article
- Keywords:
silk fibroin;
nanoparticles;
solvent displacement method;
prescription screening;
cell compatibility
- From:
Acta Pharmaceutica Sinica
2023;58(12):3715-3721
- CountryChina
- Language:Chinese
-
Abstract:
This study aimed to prepare silk fibroin nanoparticles (SF-NPs) and assess the physicochemical properties and biocompatibility of the formulation. An optimized and simplified solvent displacement method was employed to obtain SF-NPs. Single-factor prescription screening, such as silk fibroin (SF) solution concentration, the ratio of SF solution to organic solvent, ultrasonication power and time, and different types of organic phases, was used to optimize the formulation. The characterization of the optimal formulation included particle size, polydispersity index (PDI), zeta potential, morphology, and stability. The in vitro cell compatibility of the nanoparticles was evaluated using CCK-8 and Calcein-AM/PI cell viability staining. The results showed that when SF concentration was 20 mg·mL-1, volume ratio of aqueous phase to acetone was 1∶6, ultrasonic power was 80 W and ultrasonic time was 3 min, the best SF-NPs was obtained. The nanoparticles prepared in this study exhibit a near-spherical shape, with a uniform size distribution, having an average size of 144.8 nm, a PDI of 0.174, and a zeta potential of -27.35 mV. Results from in vitro cell experiments demonstrate excellent cell compatibility of SF-NPs, showing the ability to promote cell proliferation. The SF-NPs which were successfully prepared in this study exhibit uniform particle size and excellent biocompatibility.
