How autoantibodies interfere with the identification of alloantibodies
10.13303/j.cjbt.issn.1004-549x.2021.08.003
- VernacularTitle:混合抗体型自身抗体干扰同种抗体鉴定研究
- Author:
Tingwen ZHU
1
;
Rong HUANG
2
;
Fengxia LIU
2
;
Yujiao LI
1
;
Zhi YAN
1
;
Rong GUI
2
Author Information
1. Department of Blood Transfusion, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China
2. Department of Blood Transfusion, the Third Xiangya Hospital of Central South University
- Publication Type:Journal Article
- Keywords:
AIHA with both warm and cold antibodies;
alloantibodies;
specific autoantibodies;
adverse reaction to blood transfusion
- From:
Chinese Journal of Blood Transfusion
2021;34(8):813-817
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To explore a method to accurately identify the specificity of alloantibodies or autoantibodies in autoimmune hemolytic anemia (AIHA)patients with both warm and cold antibodies, so as to provide guidance for the selection of blood components. 【Methods】 Blood samples of AIHA patients with both warm and cold antibodies were screened by the direct antiglobulin testing (DAT). The plasma of patients were treated with dilution or adsorption method and the erythrocyte was dispersed for specificity identification of alloantibodies or autoantibodies.According to the results of antibody identification, appropriate phenotype of red blood cells(RBCs) were transfused to patients, and the incidence of adverse reactions and efficacy of transfusion were observed. 【Results】 Alloantibodies or specific autoantibodies were detected in serum or elution in 14 of the 16 patients. 10 patients underwent blood transfusion during hospitalization, and all of them received RBCs with the same or compatible ABO/Rh (D) type as the patients and without any reaction to the alloantibodies and specific warm autoantibodies. No hemolytic reaction occurred, and anemia symptoms were improved after blood transfusion. 【Conclusion】 The selection of appropriate methods could eliminate the influence of autoantibodies on the identification of alloantibodies in AIHA patients with both warm and cold antibodies. Therefore, the selection of blood from compatible donors for transfusion could effectively avoid the occurrence of hemolytic reaction.
