Determination of methylene blue release in plasma virus inactivation bag by fluorescence energy transfer method
10.13303/j.cjbt.issn.1004-549x.2022.03.002
- VernacularTitle:荧光能量转移法测定病毒灭活血袋中的亚甲蓝释放量
- Author:
Jing LAN
1
;
Yi GUO
1
;
Jin DING
1
;
Yuan ZHANG
1
;
Na FENG
1
;
Peng PENG
1
;
Na LIU
1
;
Xin CAO
1
;
Hua XU
1
Author Information
1. Shaanxi Blood Center, Xian 710000, China
- Publication Type:Journal Article
- Keywords:
BSA-stabilized gold nanoclusters;
fluorescence energy transfer;
virus inactivation bag;
methylene blue release
- From:
Chinese Journal of Blood Transfusion
2022;35(3):242-245
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To establish a simple, economical and rapid method for the determination of methylene blue (MB) release in virus inactivation bag. 【Methods】 Based on the fluorescence energy transfer between MB and BSA-stabilized gold nanoclusters (BSA-AuNCs), the standard curve of MB determination was established by measuring the fluorescence quenching degree of MB to BSA-AuNCs in different concentrations to conduct the determination of MB release in virus inactivation bag. 【Results】 There was a good linear relationship between the MB concentration (cMB) and the fluorescence quenching degree of BSA-AuNCs[ (I0-I)/I0=0.018cMB+ 0.021(r=0.996)] when the fluorescence emission wavelength was about 620 nm and the cMB was in the range of (0.9-36) μmoL/L. The recovery of MB was 98.00% -101.95 % when applied to determine MB at high, medium, and low concentrations, the obtained intra-day variation coefficients were 0.73%, 0.81% and 0.77% respectively, and the obtained inter-day variation coefficients were 3.92%, 3.81%, and 4.73% respectively. There was no significant difference between the results measured by this method and those measured by combination of solid-phase extraction and spectrophotometry(P>0.05). 【Conclusion】 The fluorescence energy transfer method could achieve simple and rapid determination of MB release in virus inactivation bag with accurate and reliable results.
