Effect of low concentration of sodium fluoride on osteogenic/odontogenic differentiation of human dental pulp cells
10.12016/j.issn.2096-1456.2024.01.004
- VernacularTitle:低浓度氟化钠对人牙髓细胞的成骨/成牙本质分化的影响
- Author:
Lifen LI
1
;
Junli HAN
;
Long JIANG
Author Information
1. 上海交通大学医学院附属第九人民医院口腔综合科,上海交通大学口腔医学院,国家口腔医学中心,国家口腔疾病临床研究中心,上海市口腔医学重点实验室,上海市口腔医学研究所,上海(200011)
- Keywords:
human dental pulp cell;
sodium fluoride;
proliferation;
osteogenic/odontogenic differentiation;
endoplasmic reticulum stress;
splicing xbox binding protein 1;
activating transcription factor 4;
glucose-regulated protein 78;
RNA-activated protein kinase-like
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2024;32(1):22-28
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of low concentrations of sodium fluoride on the osteogenic/odontogenic differentiation of human dental pulp cells(hDPCs)in vitro.Methods This study was reviewed and approved by the Ethics Committee.hDPCs were cultured using a modified tissue explant technique in vitro.The effects of different con-centrations of sodium fluoride on the proliferation of hDPCs were measured by methylthiazol tetrazolium(MTT)assay.Appropriate concentrations were added to the osteogenic/odontogenic differentiation induction medium,and the cells were induced in vitro.Alizarin red S staining was used to detect the osteoblastic/odontogenic differentiation ability of the cells,and the mRNA expression of the key differentiation factors was detected by RT-qPCR.Moreover,the expres-sion of key molecules of endoplasmic reticulum stress(ERS)was detected by RT-qPCR and Western blot.The data were analyzed with the SPSS 18.0 software package.Results Low concentration of NaF(0.1 mmol/L)could stimulate cell proliferation in vitro,while a high concentration(5-10 mmol/L)could inhibit cell proliferation(P＜0.05).According to the literature and the experimental data,0.1 mmol/L NaF was selected as the following experimental concentration.The levels of alizarin red S staining were increased after NaF induction of mixed osteogenic/odontogenic differentiation in vi-tro.The mRNA expression levels of key molecules for osteogenic/odontogenic differentiation,dentin sialophosphoprotein(DSPP),bone sialoprotein(BSP)and osteocalcin(OCN),were increased(P＜0.05).The mRNA levels of ERS markers(splicing x-box binding protein-1(sXBP1),glucose-regulated protein 78(GRP78)and activating transcription Factor 4(ATF4)were increased in NaF-treated cells.The protein expression levels of key ER stress molecules(phosphorylated RNA-activated protein kinase-like ER-resident kinase(p-PERK),phosphorylated eukaryotic initiation factor-2α(p-eIF2α)and ATF4)were higher in NaF-treated cells.Conclusion A low concentration of NaF promotes the osteogenic/odontogenic differentiation of hDPCs and increases the level of ER stress.
- Full text:低浓度氟化钠对人牙髓细胞的成骨_成牙本质分化的影响.pdf
