Purpose The aim was to examine c-MET,ALK,ROS1 variants in advanced non-small cell lung cancer (NSCLC) patients,and to analysis the association of c-MET,ALK,ROS1 variants with the clinical and pathological features.Methods The c-MET,ALK,ROS1 were detected by fluorescence in situ hybridization (FISH) in the 91 cases of NSCLC specimens.The correlation of c-MET gene amplification with clinicopathological features and the ALK,ROS1 fusions was analyzed.Results The positive rate of c-MET gene amplification was 8.79％ (8/91),the positive rates on male and female were 1.82％ and 19.4％,respectively.In ＜ 60-years-old and ≥60-years-old NSCLC patients,the positive rates were 7.5％ and 8.89％,resepectively.The positive rate was higher in stage Ⅲ than stage Ⅳ (9.62％ vs 7.69％),the c-MET gene amplification was detected in 9.2％ adenocarcinoma patients but none in squamous carcinoma patients.The detection rates of ALK fusions and ROS1 fusions were 10％ and 13.3％,respectively.One patient was detected the coexistence of MET with ROS1 fusion.Conclusion The c-MET gene amplification is correlated with gender,but not with age,histological types and clinical stages.C-MET amplification,ALK fusions and ROS1 fusions are almost no coexistence,but not completely mutually exclusive.To they knowledge,this is the first case report the coexistence of MET amplification with ROS1 fusion in NSCLC.

Objective To assess the effects of hypoxia combined with heavy physical workload on cardiac function of the military personnel who stationed at high altitude and to conclude the recovery effects as the personnel returning to normal altitude. Methods Ninety-six male military personnel,who rushed to plateau (3700 m) and undertaken heavy physical work for 50 days,were divided into severe,moderate, mild and no acute high altitude reaction (AHAR) group by their AHAR symptoms.Serum creatine kinase isoenzyme-MB (CK-MB) and lactic dehydrogenase isoenzyme-1(LDH-1),myocardial performance index (Tei index),left ventricular ejection fraction (LVEF),and left ventricular fractional shortening (LVFS) were respectively measured before they returned to,12 h and 15 d after they stationed at normal altitude (1500 m).Another 50 healthy volunteers who stationed at 1500 m were chosen as control group. Results The incidence of AHAR was 74.0％(71/96),including 32.5％ (25/71) mild,31.0％ (22/71) moderate and 33.8％ (24/71) severe cases.Level of serum CK-MB,LDH-1 and Tei index of severe group were significantly higher than those of moderate,mild and no AHAR group (P＜0.01),while LVEF,LVFS were significantly lower (P＜0.01).For stationed at 3700 m for 50 d,personnel's CK-MB and LDH-1 level was positively correlated with Tei index (r=0.625,0.598,P＜0.01),and negatively correlated with LVEF and LVFS (r=-0.716,-0.658,-0.639,-0.727,P＜0.01).The CK-MB and LDH-1 level and Tei index that gathered for 50-d stay were significantly higher than those of theirs stationed at 1500 m for 12 h and 15 d respectively,as well as higher than those of control group,but LVEF and LVFS were significantly lower in same comparison (t =7.146-28.613,P＜ 0.01).The self comparisons between 12 h and 15 d stationings respectively in AHAR and control group showed significant differences (t=8.677-17.852,P＜0.01).For staying 15 d at 1500 m,parameters showed no significant difference with control group's (t=0.612-1.558,P＞0.05). Conclnsions Hypoxia with heavy physical workload could obviously cause military personnel's cardiac function impairment and the harm is aggravated with the severity of AHAR.The cardiac function would obviously improve as returning to normal altitude for 12 h,and 15-d recovery would enable cardiac function normalized.

Objective To assess the effects of hypoxia combined with heavy physical workload on cardiac function of the military personnel who stationed at high altitude and to conclude the recovery effects as the personnel returning to normal altitude. Methods Ninety-six male military personnel,who rushed to plateau (3700 m) and undertaken heavy physical work for 50 days,were divided into severe,moderate, mild and no acute high altitude reaction (AHAR) group by their AHAR symptoms.Serum creatine kinase isoenzyme-MB (CK-MB) and lactic dehydrogenase isoenzyme-1(LDH-1),myocardial performance index (Tei index),left ventricular ejection fraction (LVEF),and left ventricular fractional shortening (LVFS) were respectively measured before they returned to,12 h and 15 d after they stationed at normal altitude (1500 m).Another 50 healthy volunteers who stationed at 1500 m were chosen as control group. Results The incidence of AHAR was 74.0％(71/96),including 32.5％ (25/71) mild,31.0％ (22/71) moderate and 33.8％ (24/71) severe cases.Level of serum CK-MB,LDH-1 and Tei index of severe group were significantly higher than those of moderate,mild and no AHAR group (P＜0.01),while LVEF,LVFS were significantly lower (P＜0.01).For stationed at 3700 m for 50 d,personnel's CK-MB and LDH-1 level was positively correlated with Tei index (r=0.625,0.598,P＜0.01),and negatively correlated with LVEF and LVFS (r=-0.716,-0.658,-0.639,-0.727,P＜0.01).The CK-MB and LDH-1 level and Tei index that gathered for 50-d stay were significantly higher than those of theirs stationed at 1500 m for 12 h and 15 d respectively,as well as higher than those of control group,but LVEF and LVFS were significantly lower in same comparison (t =7.146-28.613,P＜ 0.01).The self comparisons between 12 h and 15 d stationings respectively in AHAR and control group showed significant differences (t=8.677-17.852,P＜0.01).For staying 15 d at 1500 m,parameters showed no significant difference with control group's (t=0.612-1.558,P＞0.05). Conclnsions Hypoxia with heavy physical workload could obviously cause military personnel's cardiac function impairment and the harm is aggravated with the severity of AHAR.The cardiac function would obviously improve as returning to normal altitude for 12 h,and 15-d recovery would enable cardiac function normalized.