OBJECTIVETo study major antioxidant active compounds in Mori Ramulus.

METHODThe combination of on-line HPLC method and liquid chromatography-mass spectrometry was used to identify major antioxidant compounds and their content was determined by HPLC-DAD (detection wavelength of 320 nm).

RESULTOxyresveratrol was proved to be the major antioxidant compound in Mori Ramulus ethanolic extracts. Using the HPLC-DAD quantitative determination, Mori Ramulus oxyresveratrol had a good linear range of 14-1 260 mg x L(-1) (r = 0.999 96). The average recovery was 98.2% with RSD of 1.2%. This method is simple, rapid, accurate and sensitive. There was rich oxyresveratrol in Mori Ramulus and the content was significantly different according to the mulberry varieties.

CONCLUSIONThe on-line HPLC-MS-DPPH method is applicable for the determination of antioxidant compounds in Mori Ramulus.

Antioxidants ; analysis ; chemistry ; isolation & purification ; Chromatography, High Pressure Liquid ; methods ; Drugs, Chinese Herbal ; chemistry ; pharmacology ; toxicity ; Mass Spectrometry ; Medicine, Chinese Traditional ; Morus ; chemistry ; Plant Extracts ; analysis ; chemistry ; isolation & purification ; Plant Leaves ; chemistry ; Reproducibility of Results ; Sensitivity and Specificity ; Stilbenes ; analysis ; chemistry ; isolation & purification ; Time Factors