Objective To screen the key N6-methyladenosine(m6A)methylation related genes in renal clear cell carcinoma(ccRCC),and to study their expression and relationship with the prognosis,migration and invasion of renal clear cell carcinoma.Methods The RNA sequencing data and clinical data of ccRCC and ad-jacent tissues were downloaded from the Cancer Genome Atlas(TCGA)and GTEx(Genotype-Tissue Expres-sion).The expression profile and prognosis were analyzed with R 4.1.1,and the key genes were screened.Clinical specimens of 10 patients with ccRCC were collected.The mRNA and protein expressions were detec-ted by RT-qPCR and immunohistochemistry,respectively.In human ccRCC cell line RCC23,siRNA was used to knock down key genes,and CCK-8 was used to detect the survival rate of cells.Scratch test and Trans well test were used to detect the migration and invasion of cells,respectively.Results Among the 19 m6A methyl-ation related genes,only insulin-like growth factor 2 mRNA binding protein 3(IGF2BP3)was highly ex-pressed in cancer tissues,and the high expression was significantly positively correlated with poor prognosis.The high expression of IGF2BP3 was verified in clinical specimens by RT-qPCR and immunohistochemistry.After knockdown of IGF2BP3 by siRNA,the survival rate of RCC23 cells decreased significantly,and the mi-gration and invasion ability of cut cells decreased.Conclusion These results suggest that IGF2BP3 may be an effective biomarker and potential drug target for predicting the prognosis of patients with ccRCC.

Objective To explore the high-risk factors and related prevention and treatment strategies of urosepsis after transurethral flexible ureteroscopic holmium laser lithotripsy.Methods A retrospective analysis was conducted on 641 patients who underwent flexible ureteroscopic holmium laser lithotripsy in the Department of Urology,Guangyuan Central Hospital from January 2018 to December 2022.The clinical data of 42 patients with different degrees of urosepsis after surgery were collected and analyzed.Results The proportion of postoperative complications of urogenic sepsis was 6.55％(42/641).Patients underwent postoperative blood routine tests at 2h and 12h,procalcitonin indicators,electrocardiographic monitoring of vital signs,and early observation and recognition by medical staff to predict and detect signs of sepsis in a timely manner.Through active early use of potent antibiotics to combat infection,shock,and other comprehensive treatments,all patients were cured.Conclusion Analysis show that women,advanced age,poor constitution,combined with diabetes,immune system diseases(long-term use of hormones),and underlying diseases such as cardiopulmonary insufficiency.Preoperative urine culture(+)may be a high-risk factor for postoperative sepsis.Therefore,reasonable individualized treatment plans and careful preoperative preparations before surgery,skilled and meticulous operations during surgery,reasonable control of water pressure,and good control of operation time,early identification after surgery and timely and effective intervention are extremely important.