Objective To investigate how tumor-associated macrophages(TAMs)in cervical cancer cells respond to AMPK through phenotypic transformation.Methods RT-qPCR and Western blot were used to detect AMPK expression in cervical cancer tissues and HeLa,SiHa and Caski cell lines.The AMPK knockout cell line of HeLa cells was generated using the siRNA technique.The malignant biological behavior of cancer cells mediated by the AMPK/CCL18 axis was investigated using CCK-8,scratch assay and Transwell assay.Cell conditioned medium from each group was collected,and cervical cancer cell conditioned medium was co-cultured with human mononuclear cells(THP-1)to assess THP-1 recruitment and the expression of macrophage typing related markers.The impact of AMPK on tumor proliferation was also evaluated in nude mice.Results QRT-PCR and Western blot analysis showed that the mRNA(1.78±0.30)and protein(1.71±0.33)expression levels of AMPK in cancer tissues were significantly higher than those in adjacent tissues(1.00±0.10,1.00±0.11),and the differences were statistically significant(t=5.966,4.990,all P<0.01).Compared with normal cervical epithelial cells(HUCEC),the mRNA and protein expression of AMPK were significantly increased in three cell lines(HeLa,SiHa,Caski),and the differences were statistically significant(F=5.958,3.457,all P<0.01).Compared with the NC group,knocking down AMPK inhibited the proliferation of cervical cancer cells(1.33±0.18 vs 0.82±0.25),migration(180.07±7.64 vs 126.98±29.00),and invasion(115.90±13.19 vs 68.61±12.87)in cervical cancer cells,with statistical significance(t=4.015,4.337,6.285,all P<0.01).The study found that THP-1 cells exhibited significant reductions in migration(49.34±3.91 vs 33.96±10.94),invasion(28.54±3.06 vs 19.54±6.16)and M2-type polarization(1.01±0.13 vs 0.55±0.11)when AMPK reduction was induced,and these differences were statistically significant(t=3.242,3.207,6.510,all P<0.01).In vivo experiments further supported these findings,showing that AMPK reduction led to significant decreases in tumor volume(721.56±93.70mm3 vs 520.02±47.54mm3)and weight(1.19±0.06g vs 0.86±0.20g),with statistically significant differences(t=4.289,3.582,P<0.01).Conclusion Through modulating M2 polarization in TAMs,the AMPK/CCL18 axis increases cervical cancer cells'proliferation,migration and invasion.AMPK/CCL18 inhibition could provide novel therapeutic targets and approaches for cervical cancer treatment.

Objective To investigate how tumor-associated macrophages(TAMs)in cervical cancer cells respond to AMPK through phenotypic transformation.Methods RT-qPCR and Western blot were used to detect AMPK expression in cervical cancer tissues and HeLa,SiHa and Caski cell lines.The AMPK knockout cell line of HeLa cells was generated using the siRNA technique.The malignant biological behavior of cancer cells mediated by the AMPK/CCL18 axis was investigated using CCK-8,scratch assay and Transwell assay.Cell conditioned medium from each group was collected,and cervical cancer cell conditioned medium was co-cultured with human mononuclear cells(THP-1)to assess THP-1 recruitment and the expression of macrophage typing related markers.The impact of AMPK on tumor proliferation was also evaluated in nude mice.Results QRT-PCR and Western blot analysis showed that the mRNA(1.78±0.30)and protein(1.71±0.33)expression levels of AMPK in cancer tissues were significantly higher than those in adjacent tissues(1.00±0.10,1.00±0.11),and the differences were statistically significant(t=5.966,4.990,all P<0.01).Compared with normal cervical epithelial cells(HUCEC),the mRNA and protein expression of AMPK were significantly increased in three cell lines(HeLa,SiHa,Caski),and the differences were statistically significant(F=5.958,3.457,all P<0.01).Compared with the NC group,knocking down AMPK inhibited the proliferation of cervical cancer cells(1.33±0.18 vs 0.82±0.25),migration(180.07±7.64 vs 126.98±29.00),and invasion(115.90±13.19 vs 68.61±12.87)in cervical cancer cells,with statistical significance(t=4.015,4.337,6.285,all P<0.01).The study found that THP-1 cells exhibited significant reductions in migration(49.34±3.91 vs 33.96±10.94),invasion(28.54±3.06 vs 19.54±6.16)and M2-type polarization(1.01±0.13 vs 0.55±0.11)when AMPK reduction was induced,and these differences were statistically significant(t=3.242,3.207,6.510,all P<0.01).In vivo experiments further supported these findings,showing that AMPK reduction led to significant decreases in tumor volume(721.56±93.70mm3 vs 520.02±47.54mm3)and weight(1.19±0.06g vs 0.86±0.20g),with statistically significant differences(t=4.289,3.582,P<0.01).Conclusion Through modulating M2 polarization in TAMs,the AMPK/CCL18 axis increases cervical cancer cells'proliferation,migration and invasion.AMPK/CCL18 inhibition could provide novel therapeutic targets and approaches for cervical cancer treatment.

Objective To study the single nucleotide polymorphisms (SNP) in 3 sites allele (T189M, R85H, C121W) of SCN1B and the association between gene distribution and epilepsy. Methods All 330 blood samples of refractory (80 cases), non-refractory (100 cases) epilepsy patients and healthy people (150 cases) were collected. Genomic DNA of leucocyte was extracted. SNPs of three sites allele of SCN1B were tested by allele-specific primer-polymerase chain reaction (ASP-PCR).Data were analyzed by SAS 8.1 statistical software. Results Epilepsy group and healthy group had significantly statistical difference in composition of 3 sites allele on single site genotype (x~2=11.19, 11.14 and 6.50, all P < 0.05).There was no statistical significance between refractory and non-refractory epilepsy group. On gene combination, in 27 different combinations of polymorphism, mutation frequency in 3 sites (CT + AG + CG) was highest in epilepsy group (18.40%).The next was one site in CT + GG + CC (16.80%).In healthy group, frequency of non-variant in CC + GG + CC was highest (16.67%), and the next was 2 sites in CT+ AG+CC (13.73%).Thirty-five cases in epilepsy group (28.80%) had 3 sites mutation compared with 10 cases in healthy group (9.71%), and their difference had statistical significance (x~2=12.54, P<0.05).Eighteen cases in refractory epilepsy group (30.51%) had 3 sites mutation compared with 21 cases in non-refractory epilepsy group (28.77%), and the difference had no statistical significance. Fifty cases in epilepsy group (40.00%) had 2 sites mutation compared with 41 cases in healthy group (40.20%), and there was no statistical significance between them; 25 cases in refractory epilepsy group (42.37%) had 2 sites mutation compared with 21 cases in non-refractory epilepsy group (28.71%), and their difference had no statistical significance. Conclusions Mutation, especially multisite mutation of SCN1B is relatively likely to cause epilepsy in human. Gene distribution and combination of three sites allele of SCN1B in refractory epilepsy is close to that in non-refractory epilepsy.

On the basis of analyzing the defects in traditional averaging theory for extracting evoked potential (EP), and by realizing the characteristic of spontaneous electroencephalo-signal (S-EES) as well as the special environment for extracting EP, we propose an auto-reference, auto-correlation, adaptive interference cancellation (AAA-ICT) for use in the single trial of flash visual evoked potential (FVEP). Firstly, the segment of reference signal, which has the best correlation with evoked electroencephalo-signal (E-EES), was obtained using the method for calculating the sliding correlation point by point between E-EES and reference signal; then, the cancellation factor between E-EES and the most correlative reference signal segment was derived by the least square method; at last, the single trial of FVEP was acquired by interference cancellation. By this method, FVEP can be extracted perfectly and the FVEP variability of individual inter-stimulation can be obtained.
Algorithms ; Electroencephalography ; methods ; Evoked Potentials, Visual ; physiology ; Humans ; Signal Processing, Computer-Assisted

Objective To study the distribution patterns of the SNPs for the 3 sites (Ⅱe105Val, Ala114Val and Asp147Tyr) of glutathione S-transferase pi (GST-pi) in epilepsy patients without definite etiological factors. Methods At the same time, the possible relationship of GST-pi gene mutation with the vulnerability of drug-resistant epilepsy, drug-responsive epilepsy and EEG feature were explored. The SNPs of GST-pi for healthy people, drug-responsive epilepsy patients and drug-resistant epilepsy patients were genotyped by sequence-specific primers (SSP)-based PCR technologies (PCR-SSP). Results In drugresponsive epilepsy group, the frequency for 3 sites of mutated SNP of GST-pi was 59.62%, 55.32% and 50.94%, while it was 58.33%, 51.19% and 45.92% in drug-resistant epilepsy group. The difference of genotype and allele between normal group and foregoing epilepsy group was significant ( P<0.01 ), but no difference was found between drug-respensive epilepsy group and drug-resistant epilepsy group ( P>0.05 ). There was a difference of genotype distribution between groups with typical and untypical epilepsy EEG ( F = 0.0294, 8.867 × 10-6, 1.366 × 10-5, P<0.05 ). Conclusions The results indicate that the SNPs of GST-pi are associated with an increased risk of epilepsy, but not associated with an increased risk of drugresistant epilepsy. The patients present EEG characteristic of typical epilepsy.

The vehicle-mounted wireless tele-consultation system involves computer network technology, satellite communication technology and multimedia video technique in, which is composed of GPS, vehicle -mounted satellite communication sub-system, automatic satellite-tracking sub-system and computer control system. The system can be applied to medical command, operation tele-instruction, tele-education, tele -consultation, uploading medical service data and searching electronic medical information,and thus the field medical treatment and medical service are both improved. This paper introduces such aspects of the system as its principle, components, functions and application to medical service support.