Effective annotation of in vivo drug metabolites using liquid chromatography-mass spectrometry (LC-MS) remains a formidable challenge. Herein, a metabolic reaction-based molecular networking (MRMN) strategy is introduced, which enables the "one-pot" discovery of prototype drugs and their metabolites. MRMN constructs networks by matching metabolic reactions and evaluating MS² spectral similarity, incorporating innovations and improvements in feature degradation of MS² spectra, exclusion of endogenous interference, and recognition of redundant nodes. A minimum 75% correlation between structural similarity and MS² similarity of neighboring metabolites was ensured, mitigating false negatives due to spectral feature degradation. At least 79% of nodes, 49% of edges, and 97% of subnetworks were reduced by an exclusion strategy of endogenous ions compared to the Global Natural Products Social Molecular Networking (GNPS) platform. Furthermore, an approach of redundant ions identification was refined, achieving a 10%-40% recognition rate across different samples. The effectiveness of MRMN was validated through a single compound, plant extract, and mixtures of multiple plant extracts. Notably, MRMN is freely accessible online at https://yaolab.network, broadening its applications.

Objective To detect plasma renin activity(PRA) and kidney damage in primary hyperaldosteronism(PA). Methods The duration of hypertension,serum creatinine and aldosterone levels,recumbent and upright PRA levels were detected and studied in 76 PA patients.Correlation was measured between creatinine and other parameters. Results Serum creatinine level correlates closely with upright PRA ( P

Purpose:To evaluate the surgical method of pheochromocytoma.Methods:Data of 103 cases of patients with pheochromocytoma was reviewed retrospectively.Results:The operation of 49 patients with Pheochromocytoma were performed by the 11 th rib intercostal incision, 18 cases by the 10th rib intercostal incision, 15 cases by transrectus incision, 9 cases by abdominothoracic incision, 5 cases by abdominal subcostal oblique, 3 cases by inferior abdominal incision, 3 cases by posterolateral incision. 1 patient received the laparoscopic surgery.Conclusions:① Most surgical pathway of the pheochromocytoma in adrenal can be performed by the 11th rib intercostal incision of the 10th rib intercostal incision.②The suitable pathways for the giant pheochromocytoma adhering to big vessels are transrectus incision or abdominothoracic incision.③ For the mutiple masses of extra-adrenal pheochromocytoma, the middle abdominal incision may be the choice.

Objective To probe into the diagnosis and treatment of pheochromocytoma of high risk. Methods The clinical data of 35 cases of pheochromocytoma of high risk underwent surgical extirpation from 1990 to 2000 were reviewed. Results The tumors were intraadrenal in 27 and extraadrenal in 8.The average diameter of the growth which could be successfully resected was 10 cm and the weight 350 g.Biopsy was undertaken in 2 because of the difficulty in extirpation of the tumor.Recurrence occurred in 4 and there were 3 cases being comfirmed to be malignant. Conclusions Adequate preoperative preparation and definite location of the tumor are the most important before surgery.Fluctuation of blood pressure have to be monitored during surgery and to be treated carefully and promptly.Hypoglycemia have to be awared of after the surgery.