ObjectiveThis study aims to conduct a 28-day oral toxicity test to observe the subacute toxic effects of Ganoderma lucidum spore oil in rats, elucidate its impacts on physiological indicators in rats, and provide a scientific basis for the safety evaluation of this substance. MethodsEighty 4-week-old specific pathogen-free (SPF) SD rats were randomly divided into a control group and three dose groups: low-dose (0.83 g/kg), medium-dose (1.67 g/kg), and high-dose (3.33 g/kg), with 10 males and 10 females in each group. The experimental groups were given Ganoderma lucidum spore oil by gavage at the corresponding doses, while the control group was given the same volume of edible corn oil. Administration lasted for 28 consecutive days, during which the rats had free access to food and water. The general condition of the rats was observed, and body weight, food intake, and food utilization rate were measured. After the treatment period, urine was collected from rats in each group for routine urinalysis. Blood samples were collected from the abdominal aorta under anesthesia induced by intraperitoneal administration of Zoletil 50 (5 mg/kg) and xylazine hydrochloride (10 mg/kg) for complete blood count, coagulation testing, and serum biochemical analyses. Gross necropsy was performed in all rats. Organs were excised and weighed, organ coefficients of major organs were calculated, and histopathological examinations of major organs were conducted in the control and high-dose groups. ResultsDuring the entire experimental period, all rats in the control and the three dose groups thrived without any mortality. No abnormal behaviors or clinical signs were observed in any of the groups. In the serum biochemistry, a statistically significant increase (P<0.05) in total protein was found in the high-dose female group compared with the control group. However, there was no dose-response relationship, and the values remained within the normal range of historical data in our laboratory; therefore, the increase was considered not biologically significant. Rats in the remaining groups showed no statistically significant differences (all P>0.05) in complete blood count, serum biochemical parameters, coagulation parameters, urinalysis parameters, or organ coefficients compared with the control group. Histopathological examination revealed no significant abnormalities. ConclusionThe results indicate that Ganoderma lucidum spore oil does not exhibit significant subacute toxicity in SD rats at the tested dose levels.

Objective To investigate the effect of ginkgolide B(BN52021) on the NF-?B expression in pulmonary tissue of mice with LPS-induced acute lung injury.Methods Fifty Kunming mice were divided into 2 groups at random: LPS group(L group) and BN52021 pretreated group(B group),and then each group was subsequently divided into 5 subgroups according to different time point(0,1,3,9 and 12 h).The mice from corresponding groups received an intraperitoneal injection of normal saline or 20 mg/kg BN52021 followed by an injection of 15 mg/kg LPS 5 min later.The mice were sacrificed in 0,1,3,9 and 12 h after LPS injection.The wet/dry ratio(W/D) was recorded for pulmonary function and the pathological changes of lung tissues stained by HE were examined by light microscopy.The expression of NF-?B p65 was detected by Western blotting,and the levels of tumor necrosis factor-?(TNF-?) and interleukin-10(IL-10) were determined by ELISA.Results The significant increase of W/D was found after LPS injection in L group,which indicated the existence of pulmonary edema;while the W/D in B group was lower(P