Objective To explore the feasibility of applying artificial intelligence (AI) technology in chromosomal aberration (CA) analysis for occupational health surveillance of radiation workers and in biological dose estimation during nuclear emergency responses. Methods Peripheral blood samples from healthy volunteers were irradiated in vitro with X-rays and cobalt-60 (⁶⁰Co) γ rays. Chromosome slides were prepared using an automated harvesting and dropping device. The data training and outcome evaluation of CA analysis was performed on the AI software using chromosome images from occupational medical examination of radiation workers from the current lab or chromosome slides from blood samples irradiated with X-rays. The trained AI software was then used to assist in CA analysis and biological dose estimation among occupational medical examination of radiation workers, with results compared with manual reading and actual exposure doses. Results The trained AI software achieved a CA recognition accuracy of 95.11%. In the occupational health examination of radiation workers, the positive CA detection rate using AI + manual review was 2.25% higher than that in manual reviewing alone. The errors in biological dose estimation for ⁶⁰Co γ rays and X-rays using AI + manual review analysis were 11.86% and 7.33%, respectively, both within the acceptable 20.00% error margin. Conclusion AI + manual review can be effectively applied in CA analysis for occupational health examination and biological dose estimation during nuclear emergencies, significantly improving analysis efficiency.

Objective To explore the surgical techniques and therapeutic effects of hematoma evacuation for hypertensive cerebral hemorrhage(HCH)with different bleeding sites under neuroendoscopy.Methods This study enrolled 101 patients with HCH treated in our hospital from May 2022 to January 2024.Based on CT imaging results,patients were divided into lobar cerebral hemorrhage group(n=43)and basal ganglia hemorrhage group(n=58).The Pearson was used to analyze the correlations between cerebral microcirculation indicators and the scores of national institutes of health stroke scale(NIHSS),mini mental state examination(MMSE),and the Barthel index(BI)of activities of daily living.The generalized estimating equation was employed to analyze the improvement effects of neuroendoscopic hematoma evacuation on cerebral microcirculation indicators in patients with different hemorrhage locations.A difference-in-differences equation model with full specifications was applied to analyze the improvement effects of neuroendoscopic hematoma evacuation on NIHSS score,MMSE score,and BI score in patients with different hemorrhage locations,incorporating baseline indicators as control variables.Results The operative time of basal ganglia hemorrhage group was significantly longer than those of lobar cerebral hemorrhage group,and the differences of NIHSS,MMSE,BI,mean transit time(MTT),cerebral blood flow(CBF),cerebral blood volume(CBV)and mean arterial pressure(MAP)before and after treatment were significantly smaller than those of lobar cerebral hemorrhage group,the differences were statistically significant(P<0.05).There was no significant difference in the incidence of complications between the two groups(P>0.05).The correlation analysis results showed that MTT was positively correlated with NIHSS,and negatively correlated with BI and MMSE;CBF,CBV and MAP were negatively correlated with NIHSS,but positively correlated with BI and MMSE,the differences were statistically significant(P<0.05).The result of generalized estimating equation analysis showed that the improvement of MTT,CBF,CBV and MAP in patients with lobar cerebral hemorrhage group was better than those in patients with basal ganglia hemorrhage group,the differences were statistically significant(P<0.05).The result of difference-in-differences equation model analysis showed that increase of age,prolongation of MTT and prolongation of the time from onset to operation had positive effects on NIHSS score(B=0.884,1.291,0.758,P<0.05),and had negative effects on MMSE score(B=-1.014,-1.569,-0.821,P<0.05).The prolongation of MTT had a negative effect on BI score(B=-0.973,P<0.05).The increase of CBV,CBF and MAP had a negative effect on NIHSS score(B=-0.841,-0.767,-1.213,P<0.05),and had a positive effect on MMSE and BI score(MMSE:B=0.932,0.738,0.874;BI:B=0.897,0.751,0.842,P<0.05).Conclusion Neuroendoscopic hematoma evacuation can improve the NIHSS score,MMSE score,BI score of patients with HCH by adjusting MTT,CBF,CBV and MAP,and the improvement effect of patients with lobar cerebral hemorrhage is significantly better than that of patients with basal ganglia hemorrhage.

Objective To explore the surgical techniques and therapeutic effects of hematoma evacuation for hypertensive cerebral hemorrhage(HCH)with different bleeding sites under neuroendoscopy.Methods This study enrolled 101 patients with HCH treated in our hospital from May 2022 to January 2024.Based on CT imaging results,patients were divided into lobar cerebral hemorrhage group(n=43)and basal ganglia hemorrhage group(n=58).The Pearson was used to analyze the correlations between cerebral microcirculation indicators and the scores of national institutes of health stroke scale(NIHSS),mini mental state examination(MMSE),and the Barthel index(BI)of activities of daily living.The generalized estimating equation was employed to analyze the improvement effects of neuroendoscopic hematoma evacuation on cerebral microcirculation indicators in patients with different hemorrhage locations.A difference-in-differences equation model with full specifications was applied to analyze the improvement effects of neuroendoscopic hematoma evacuation on NIHSS score,MMSE score,and BI score in patients with different hemorrhage locations,incorporating baseline indicators as control variables.Results The operative time of basal ganglia hemorrhage group was significantly longer than those of lobar cerebral hemorrhage group,and the differences of NIHSS,MMSE,BI,mean transit time(MTT),cerebral blood flow(CBF),cerebral blood volume(CBV)and mean arterial pressure(MAP)before and after treatment were significantly smaller than those of lobar cerebral hemorrhage group,the differences were statistically significant(P<0.05).There was no significant difference in the incidence of complications between the two groups(P>0.05).The correlation analysis results showed that MTT was positively correlated with NIHSS,and negatively correlated with BI and MMSE;CBF,CBV and MAP were negatively correlated with NIHSS,but positively correlated with BI and MMSE,the differences were statistically significant(P<0.05).The result of generalized estimating equation analysis showed that the improvement of MTT,CBF,CBV and MAP in patients with lobar cerebral hemorrhage group was better than those in patients with basal ganglia hemorrhage group,the differences were statistically significant(P<0.05).The result of difference-in-differences equation model analysis showed that increase of age,prolongation of MTT and prolongation of the time from onset to operation had positive effects on NIHSS score(B=0.884,1.291,0.758,P<0.05),and had negative effects on MMSE score(B=-1.014,-1.569,-0.821,P<0.05).The prolongation of MTT had a negative effect on BI score(B=-0.973,P<0.05).The increase of CBV,CBF and MAP had a negative effect on NIHSS score(B=-0.841,-0.767,-1.213,P<0.05),and had a positive effect on MMSE and BI score(MMSE:B=0.932,0.738,0.874;BI:B=0.897,0.751,0.842,P<0.05).Conclusion Neuroendoscopic hematoma evacuation can improve the NIHSS score,MMSE score,BI score of patients with HCH by adjusting MTT,CBF,CBV and MAP,and the improvement effect of patients with lobar cerebral hemorrhage is significantly better than that of patients with basal ganglia hemorrhage.

Objective:To observe the influence of wogonin on colitis mice induced by dextran sulfate sodium (DSS) and explore the related mechanism.Methods:Eighteen C57BL/6 mice were randomly and equally divided into the control group, the model group and the treatment group. The water was given normally to mice in control group, and the 2.5% DSS drinking water was given to mice of other two groups for 7 days. Wogonin via intraperitoneal injection was administrated in the mice of treatment group on the second and the fourth day. The mice were sacrificed on the eighth day and specimens were collected. The pathological damage and inflammation degree of mice colon were evaluated by measuring the length of colon and using HE staining. Immunofluorescence was used to detect the infiltration of neutrophils in mice colon tissue. Wogonin of 25, 50, 100 μmol/L was applied to handle neutrophils from mice marrow tissue in vitro, and there was no treatment in the negative control group. The flow cytometry was used to detect the apoptosis of neutrophil. Western blot was used to detect the expressions of anti-apoptotic protein myeloid cell leukelia-1 (Mcl-1) and extracellular signal-regulated kinase (ERK) . Results:Compared with the model group, the mice colon length in the treatment group was significantly longer [ (7.80 ± 0.21) cm vs. (6.43 ± 0.10) cm, P<0.01], the pathological damage score of the colon tissue was significantly lower [ (6.83 ± 0.98) points vs. (14.33 ± 1.03) points, P<0.01], the number of infiltrative neutrophils in the colon of mice was significantly lower [ (8.52 ± 0.15) neutrophils per low power field vs. (29.43 ± 0.43) neutrophils per low power field, P<0.01]. The apoptosis rate of neutrophils were 6.41% ± 0.51%, 14.01% ± 0.81%, 20.89% ± 0.82%, 24.23% ± 0.29% in negative control group and 25, 50, 100 μmol/L wogonin groups. The apotosis rate of neutrophils increased constantly with the concentration of wogonin increasing gradually and there were significant differences among any two groups ( P<0.01) . Compared with the negative control group, the phosphorylated ERK expressions of neutrophils in 25, 50, 100 μmol/L wogonin groups were decreased obviously (all P<0.05) . The Mcl-1 expression of neutrophils declined constantly with the concentration of wogonin increasing gradually. Conclusion:Wogonin can induce the apoptosis of neutrophils in concentration-dependent manner, reduce the infiltration of neutrophils and relieve the intestinal damage in colon tissue of colitis mice, which may be regulated by the inhibition of ERK phosphorylation and decreased expression of Mcl-1 in concentration-dependent manner.

Objective:To observe the influence of wogonin on colitis mice induced by dextran sulfate sodium (DSS) and explore the related mechanism.Methods:Eighteen C57BL/6 mice were randomly and equally divided into the control group, the model group and the treatment group. The water was given normally to mice in control group, and the 2.5% DSS drinking water was given to mice of other two groups for 7 days. Wogonin via intraperitoneal injection was administrated in the mice of treatment group on the second and the fourth day. The mice were sacrificed on the eighth day and specimens were collected. The pathological damage and inflammation degree of mice colon were evaluated by measuring the length of colon and using HE staining. Immunofluorescence was used to detect the infiltration of neutrophils in mice colon tissue. Wogonin of 25, 50, 100 μmol/L was applied to handle neutrophils from mice marrow tissue in vitro, and there was no treatment in the negative control group. The flow cytometry was used to detect the apoptosis of neutrophil. Western blot was used to detect the expressions of anti-apoptotic protein myeloid cell leukelia-1 (Mcl-1) and extracellular signal-regulated kinase (ERK) . Results:Compared with the model group, the mice colon length in the treatment group was significantly longer [ (7.80 ± 0.21) cm vs. (6.43 ± 0.10) cm, P<0.01], the pathological damage score of the colon tissue was significantly lower [ (6.83 ± 0.98) points vs. (14.33 ± 1.03) points, P<0.01], the number of infiltrative neutrophils in the colon of mice was significantly lower [ (8.52 ± 0.15) neutrophils per low power field vs. (29.43 ± 0.43) neutrophils per low power field, P<0.01]. The apoptosis rate of neutrophils were 6.41% ± 0.51%, 14.01% ± 0.81%, 20.89% ± 0.82%, 24.23% ± 0.29% in negative control group and 25, 50, 100 μmol/L wogonin groups. The apotosis rate of neutrophils increased constantly with the concentration of wogonin increasing gradually and there were significant differences among any two groups ( P<0.01) . Compared with the negative control group, the phosphorylated ERK expressions of neutrophils in 25, 50, 100 μmol/L wogonin groups were decreased obviously (all P<0.05) . The Mcl-1 expression of neutrophils declined constantly with the concentration of wogonin increasing gradually. Conclusion:Wogonin can induce the apoptosis of neutrophils in concentration-dependent manner, reduce the infiltration of neutrophils and relieve the intestinal damage in colon tissue of colitis mice, which may be regulated by the inhibition of ERK phosphorylation and decreased expression of Mcl-1 in concentration-dependent manner.

Objective To explore the safety and effectiveness of central venous catheter for thoracic drainage after esoph-agectomy.Methods We prospectively analyzed 95 patients who underwent esophagectomy for esophageal carcinoma in our de-partment from August 2016 to August 2017.Forty-six patients with central venous catheter drainage were observation group, and 49 patients with traditional chest-tube closed drainage were control group.Compared the differences of drainage amount, pain score, postoperative patient quality of life score, duration of drainage and chest tube incision healing between the two groups.Results There were no significant differences in daily drainage volume and patency of drainage tube between the two groups(P>0.05).Compared with the control group, the duration of thoracic drainage in the observation group was significant shorter, the pain intensity score was significant lower , and the emotional function of postoperative patient quality of life was sig-nificant better(P<0.01).There were more patients suffered uncomfortable symptoms, such as nausea, vomiting, pain, in-somnia in the control group(P<0.05).Conclusion The application of central venous catheter in thoracic drainage can de-crease the postoperative pain and improve the postoperative quality of life for esophageal carcinoma patients after esophagecto-my.It is safe and feasible for thoracic drainage after esophagectomy .