Objective To explore the mediating role of mindful attention and awareness in depressive symptoms and insomnia severity among patients with comorbid depression and insomnia.Methods A cross-sectional study was conducted,enrolling 267 patients with comorbid depression and insomnia who were treated in the outpatient Department of Medical Psychology of Second Affiliated Hospital of Army Medical University,from March to May 2024.Basic demographic and clinical data were collected using a general information questionnaire.Depressive symptom severity was measured via the Patient Health Questionnaire-9(PHQ-9),insomnia severity via the Insomnia Severity Index(ISI),perceived stress via the Perceived Stress Scale-10(PSS-10),and mindful attention and awareness via the Mindful Attention Awareness Scale(MAAS).Pearson correlation analysis was used to examine the correlations between depressive severity,insomnia severity,perceived stress,and mindful attention and awareness.Mediation analysis was performed using Process 4.1.Results The PHQ-9 score was(13.80±5.98)and the ISI score was(17.10±5.56)in the 267 patients.Pearson correlation analysis showed that depressive severity and insomnia severity were positively correlated with perceived stress(r=0.531,0.351,P<0.001)and negatively correlated with mindful attention and awareness(r=-0.373,-0.350,P<0.001).Mediation analysis using Process 4.1 indicated that the combined mediating effect of mindful attention and awareness and insomnia between perceived stress level and depressive level was 0.157,with a 95%confidence interval(CI)of 0.102～0.217,and the total mediating effect was significant(P<0.001).Conclusion Perceived stress directly positively affects depression and indirectly exacerbates depression through insomnia as a mediator,and mindful attention and awareness can weaken the promoting effect of perceived stress on insomnia.

Objective:To construct an index system for including ideological and political education elements and cases in the Health Assessment course, and to provide a reference for the evaluation of ideological and political teaching of the Health Assessment course.Methods:By using literature analysis, the nominal group method, and the Delphi expert consultation method, we determined indicators for ideological and political elements and case inclusion criteria for the undergraduate nursing course Health Assessment. The analytic hierarchy method was applied to quantify the items and determine the final weights of the indicators at each level.Results:The response rates of two rounds of expert consultation were both 100%. The authority coefficient of the experts was 0.880. For the second-round consultation on the system of ideological and political elements, the coefficient of variation was 0.078, and the Kendall's coefficient of concordance was 0.334. For the second-round consultation on the inclusion criteria for ideological and political cases, the coefficient of variation was 0.075, and the Kendall's coefficient of concordance was 0.312. Finally, we determined 6 first-level indicators and 30 second-level indicators for the ideological and political elements of the Health Assessment course, and 9 indicators for the inclusion of ideological and political cases for the Health Assessment course.Conclusions:The established index system for the ideological and political elements and cases of the Health Assessment course for nursing undergraduates is reasonable, scientific, and reliable, which can provide a reference for subsequent construction of the ideological and political teaching system for the Health Assessment course.

Circulating DNA is defined as a kind of extracellular DNA that exists in plasma,cerebrospinal fluid and synovial fluid.The concentration of circulating DNA of cancer patients is significantly higher than that in healthy people.The genetic and epigenetic alterations of circulating cell-free nucleic acids are relevant to cancer development and progression,for example,gene mutation,DNA methylation and microsatellite instability and so on.The quantitative and qualitative detection of circulating DNA shows promising potential value in cancer screening,diagnosis,disease monitoring treatment and prognosis.

Objective To analyze the coping ways and influence factors of the migrant workers of the generation after 90s in Shenzhen.Methods By cluster sampling, 614 migrant workers of the generation after 90 s were surveyed with demographic data and the Simplified Coping Style questionnaire ( SCSQ ) .Results Among 614 participants the score of coping ways was (1.85 ±0.44), higher than the norm, and the negative coping score was (1.14 ±0.52), lower than the norm.The differences were statistical significant (t =-3.87,-21.34, respectively;P<0.01).There were 487 participants (79.3%) in the normal state of positive coping ways,and 376 participants (61.2%) in the normal state of negative coping ways .The education level and gender were the influence factors of coping ways , within which the education level had a significant positive effect on the coping ways (F=4.015, P=0.019), and the influence factors had a significant negative effect on the coping ways (t=3.230, P=0.001).Conclusions Most of the employee of the generation after 90 s are at a normal state of coping , and have preferable coping ways .The education level and gender are the factors that influence the ways of the behavior and reaction .The inferiority, timidity, atresia and loneliness were found among these employee during the investigation .

Objective: To establish a method for the determination of cinnamic acid in Lingguizhugan Decoction (Poria, Ramuls Cinnamomi, Rhizoma Atractylodis macrocephalae, Radix Glycrrhizae, etc.). Methods: Using RP HPLC with Hypersil C 18 column, methanol acetonitrile water acetic acid (10∶22∶70∶1.4,v/v) as mobile phase, detection wavelength at 254nm and p dimethylaminobenzaldehyde as the internal standard.Results: The mean recovery and RSD were 98.2% and 1.9% ( n =6), respectively, the linear range of cinnamic acid was 8.4～168?g?mL -1 ( r =0.9999). Conclusion: The method is simple, rapid, accurate and reproducible, and may be used for the determination of cinnamic acid in Lingguizhugan Decoction.