Objective To investigate the effect of palmitoleic acid(POA)on pyroptosis of cardiomyocytes after hy-poxia/reoxygenation-induced injury in the human myocardium.Methods The experiment comprised a control group(Control,normal culture),a hypoxia/reoxygenation group(HR),a palmitoleic acid-treated group(HR+POA),and an anhydrous ethanol control group(HR+ET).Cardiomyocytes viability was assessed using CCK-8 assay,and the level of pyroptosis of cardiomyocytes was measured through the double staining with Hoechst33342/PI and LDH assay.ELISA was employed to detect the release of inflammatory factors IL-1 β and IL-18 in the cell culture supernatant.qRT-PCR and Western blot were utilized to determine the relative expression levels of mRNA and protein of pyroptosis-related genes,namely NLRP3,ASC,Caspase-1,GSDMD,IL-1 β and IL-18,respective-ly.Results CCK-8 assay showed that the survival of hypoxic/reoxygenated cardiomyocytes increased with the ad-dition of POA at concentrations ranging from 25 to 100 μmol/L,as compared to the HR group;a hypoxia/reoxy-genation model of cardiomyocyte was established.The expression of protein and mRNA increased in NLRP3,ASC,Cleaved caspase-1,GSDMD-N,IL-Iβ and IL-18 vs the control group(P＜0.05),the positive percentage of Ho-echst33342/PI staining in cardiomyocytes increased significantly(P＜0.05),the release of LDH,IL-Iβ,and IL-18 increased(P＜0.05).After intervention with 100 μmol/L POA,the protein and mRNA expression levels of NLRP3,ASC,Cleaved caspase-1,GSDMD-N,IL-Iβ,and IL-18 were significantly reduced in the HR+POA group vs HR+ET group(P＜0.05).The positive percentage of Hoechst33342/PI staining in cardiomyocytes de-creased significantly,and the levels of LDH,IL-Iβ and IL-18 significantly decreased(P＜0.05).Conclusion Palmitoleic acid may alleviate hypoxia/reoxygenation-induced injury of cardiomyocytes by inhibiting pyroptosis and inflammatory response after hypoxia/reoxygenation in human myocardium.

In order to explore whether the expression of CircFoxO3 is related to the proliferation and apoptosis of porcine myoblasts,this study transfected porcine myoblasts with CircFoxO3 over-expression vector and siRNA,and detected cell proliferation and apoptosis by EdU and Annexin V-mCherry fluorescence staining,and detected the expression of apoptosis-related genes by RT-qPCR.The results showed that after transfection with overexpression vector,the proportion of ap-optotic cells increased and the number of cells proliferation decreased.At the same time,the ex-pression of apoptosis-related genes Caspase-3,Caspase-8,Fas and FasL was significantly up-regula-ted by RT-qPCR(P＜0.05),and the expression of Bax was extremely significantly up-regulated(P＜0.01).The expression of Bcl-2 was significantly down-regulated(P＜0.05).After transfection with si-CircFoxO3,the apoptosis level decreased and proliferation ability increased.the expression of Bax and FasL genes was highly significantly down-regulated(P＜0.01),the expression of caspase-3 and Caspase-8 was significantly decreased(P＜0.05),and the expression of Bcl-2 was significantly increased(P＜0.05).CircFoxO3 expression inhibited proliferation of porcine myo-blasts,promoted the expression of apoptosis-related genes,and promoted apoptosis of myoblasts.

In order to explore whether the expression of CircFoxO3 is related to the proliferation and apoptosis of porcine myoblasts,this study transfected porcine myoblasts with CircFoxO3 over-expression vector and siRNA,and detected cell proliferation and apoptosis by EdU and Annexin V-mCherry fluorescence staining,and detected the expression of apoptosis-related genes by RT-qPCR.The results showed that after transfection with overexpression vector,the proportion of ap-optotic cells increased and the number of cells proliferation decreased.At the same time,the ex-pression of apoptosis-related genes Caspase-3,Caspase-8,Fas and FasL was significantly up-regula-ted by RT-qPCR(P＜0.05),and the expression of Bax was extremely significantly up-regulated(P＜0.01).The expression of Bcl-2 was significantly down-regulated(P＜0.05).After transfection with si-CircFoxO3,the apoptosis level decreased and proliferation ability increased.the expression of Bax and FasL genes was highly significantly down-regulated(P＜0.01),the expression of caspase-3 and Caspase-8 was significantly decreased(P＜0.05),and the expression of Bcl-2 was significantly increased(P＜0.05).CircFoxO3 expression inhibited proliferation of porcine myo-blasts,promoted the expression of apoptosis-related genes,and promoted apoptosis of myoblasts.

Objective To study the clinical effect of adenosine triphosphate and spermatic vein ligation combined treatment of children with primary varicocele disease.Methods80 cases of children with primary varicocele disease who were treated in pediatric surgery department of Hangzhou City Yuhang District MCH from May 2014 to May 2016 were selected, these children were divided into adenosine triphosphate and spermatic vein ligation combined treatment group (combined treatment group, n= 40) and spermatic vein ligation treatment group (monotherapy group, n=40) two groups according to the random number table, the postoperative pain score, gastrointestinal recovery time, hospital stay, cost of hospitalization, incidence of complications and recurrence of the two groups were statistically analyzed.ResultsThe postoperative pain score and hospitalization cost of the combined treatment group were significantly lower (P<0.05), the gastrointestinal function recovery time and hospital stay were significantly shorter (P<0.05), the incidence of complications 2.5% (1/40) was significantly lower than the monotherapy group 17.5% (7/40) (P<0.05), the recurrence rate 2.5% (1/40) was significantly lower than the monotherapy group 10.0% (4/40) (P< 0.05).ConclusionThe clinical effect of adenosine triphosphate and spermatic vein ligation combined treatment of children with primary varicocele disease is better than spermatic vein ligation treatment alone, so is worthy of promotion in the clinical.