BACKGROUND:Mesenchymal stem cell transplantation has a certain effect on spinal cord injury,but there are still some problems such as low survival rate and poor efficiency of cell transplantation caused by local oxidative stress environment.OBJECTIVE:To explore the protective effect of mangiferin on oxidative stress of bone marrow mesenchymal stem cells.METHODS:Rat bone marrow mesenchymal stem cells at passage 3 were added to mangiferin and incubated for 2 hours according to the gradient of concentration (0,20,40,80,and 160 μmol/L).A serum-free medium containing 400 μmol/L H2O2 was added,and a gradient concentration of mangiferin was added again and cultured for 12 and 24 hours.Rat bone marrow mesenchymal stem cells were cultured as normal control group.Cell survival was detected by MTT assay in each group.The superoxide dismutase,malondialdehyde,and catalase in the culture medium were detected in accordance with the instruction of the kit.RESULTS AND CONCLUSION:Compared with normal control group,cell viability in the H2O2 group was significantly decreased (P<0.01),and the levels of superoxide dismutase and catalase were significantly decreased (P<0.01),while the level of malondialdehyde was significantly increased (P<0.01).Compared with H2O2 group,the survival rate of cells was significantly increased (P<0.01),and the levels of superoxide dismutase and catalase were significantly increased (P<0.01),while the level of malondialdehyde was significantly decreased (P<0.01) in the mangiferin group.Mangiferin showed concentration-dependent antioxidant stress protective activity above 20 μmol/L,and no cytotoxicity below 160 μmol/L.These findings indicate that antioxidant mangiferin can increase the antioxidant activity of bone marrow mesenchymal stem cells and provide a new preconditioning strategy for bone marrow mesenchymal stem cell transplantation.

BACKGROUND: The available evidence regarding the benefits of percutaneous coronary intervention (PCI) on patients receiving dialysis with coronary artery disease (CAD) is limited and inconsistent. This study aimed to evaluate the association between PCI and clinical outcomes as compared with medical therapy alone in patients undergoing dialysis with CAD in China. METHODS: This multicenter, retrospective study was conducted in 30 tertiary medical centers across 12 provinces in China from January 2015 to June 2021 to include patients on dialysis with CAD. The primary outcome was major adverse cardiovascular events (MACE), defined as a composite of cardiovascular death, non-fatal myocardial infarction, and non-fatal stroke. Secondary outcomes included all-cause death, the individual components of MACE, and Bleeding Academic Research Consortium criteria types 2, 3, or 5 bleeding. Multivariable Cox proportional hazard models were used to assess the association between PCI and outcomes. Inverse probability of treatment weighting (IPTW) and propensity score matching (PSM) were performed to account for potential between-group differences. RESULTS: Of the 1146 patients on dialysis with significant CAD, 821 (71.6%) underwent PCI. After a median follow-up of 23.0 months, PCI was associated with a 43.0% significantly lower risk for MACE (33.9% [ n  = 278] vs . 43.7% [ n  = 142]; adjusted hazards ratio 0.57, 95% confidence interval 0.45-0.71), along with a slightly increased risk for bleeding outcomes that did not reach statistical significance (11.1% vs . 8.3%; adjusted hazards ratio 1.31, 95% confidence interval, 0.82-2.11). Furthermore, PCI was associated with a significant reduction in all-cause and cardiovascular mortalities. Subgroup analysis did not modify the association of PCI with patient outcomes. These primary findings were consistent across IPTW, PSM, and competing risk analyses. CONCLUSION This study indicated that PCI in patients on dialysis with CAD was significantly associated with lower MACE and mortality when comparing with those with medical therapy alone, albeit with a slightly increased risk for bleeding events that did not reach statistical significance.
Humans ; Percutaneous Coronary Intervention/methods* ; Male ; Female ; Coronary Artery Disease/drug therapy* ; Retrospective Studies ; Renal Dialysis/methods* ; Middle Aged ; Aged ; China ; Proportional Hazards Models ; Treatment Outcome

BACKGROUND:Mesenchymal stem cell transplantation has a certain effect on spinal cord injury,but there are still some problems such as low survival rate and poor efficiency of cell transplantation caused by local oxidative stress environment.OBJECTIVE:To explore the protective effect of mangiferin on oxidative stress of bone marrow mesenchymal stem cells.METHODS:Rat bone marrow mesenchymal stem cells at passage 3 were added to mangiferin and incubated for 2 hours according to the gradient of concentration (0,20,40,80,and 160 μmol/L).A serum-free medium containing 400 μmol/L H2O2 was added,and a gradient concentration of mangiferin was added again and cultured for 12 and 24 hours.Rat bone marrow mesenchymal stem cells were cultured as normal control group.Cell survival was detected by MTT assay in each group.The superoxide dismutase,malondialdehyde,and catalase in the culture medium were detected in accordance with the instruction of the kit.RESULTS AND CONCLUSION:Compared with normal control group,cell viability in the H2O2 group was significantly decreased (P<0.01),and the levels of superoxide dismutase and catalase were significantly decreased (P<0.01),while the level of malondialdehyde was significantly increased (P<0.01).Compared with H2O2 group,the survival rate of cells was significantly increased (P<0.01),and the levels of superoxide dismutase and catalase were significantly increased (P<0.01),while the level of malondialdehyde was significantly decreased (P<0.01) in the mangiferin group.Mangiferin showed concentration-dependent antioxidant stress protective activity above 20 μmol/L,and no cytotoxicity below 160 μmol/L.These findings indicate that antioxidant mangiferin can increase the antioxidant activity of bone marrow mesenchymal stem cells and provide a new preconditioning strategy for bone marrow mesenchymal stem cell transplantation.

BACKGROUND:Hyperbaric oxygen (HBO) therapy can aleviate the skin flap congestion by improving the angiogenesis and increasing the oxygen content of blood in skin flaps. Although the HBO therapy ability to increase flap survival has been wel described, the research on the application of HBO pretreatment in skin flap transplantation does not arouse adequate concern.
OBJECTIVE: To investigate the effect of HBO pretreatment on early-stage flap congestion in the rat model of over-length dorsal random skin flaps.
METHODS: Thirty-six SD rats were randomly divided into control group (n=12), HBO pretreatment group (n=12) and HBO treatment group (n=12). Rats in the HBO pretreatment group received 4 days of HBO therapy prior to transplantation, once a day. Rats in the HBO treatment group received 4 days of HBO therapy after transplantation. Rats in the control group were raised in the normal conditions after flap transplantation. At postoperative days 3 and 5, rats were sacrificed and the samples were colected. The inflammation of flap tissues was detected using hematoxylin-eosin staining. The expression of vascular endothelial growth factor and transforming growth factor-β was analyzed by immunohistochemistry staining. The flap survival rate was calculated at postoperative day 5.
RESULTS AND CONCLUSION:The flap survival area of the HBO pretreatment group and HBO treatment group was larger than that of the control group (P < 0.05). At postoperative days 3 and 5, the expression levels of vascular endothelial growth factor and transforming growth factor-β in the flap tissue were higher in the HBO pretreatment group and HBO treatment group than the control group (P < 0.05). However, there was no significant difference in the flap survival area and the expression of vascular endothelial growth factor and transforming growth factor-β between HBO pretreatment and HBO treatment groups (P > 0.05). HBO pretreatment can increase the expression of vascular endothelial growth factor and transforming growth factor-β and promotes angiogenesis in random pattern flaps,thereby improving skin flap survival.