OBJECTIVES: To investigate the expression of apolipoprotein C1 (APOC1) in papillary thyroid carcinoma (PTC) and its effects on proliferation and apoptosis of PTC cells. METHODS: The expression level of APOC1 in PTC and its impact on prognosis were analyzed using GEPIA 2 and Kaplan-Meier databases. Immunohistochemistry (IHC) and Western blotting were used to detect the expression of APOC1 in PTC and adjacent tissues and in 3 PTC cell lines and normal thyroid Nthyori 3-1 cells. In TPC-1 and BCPAP cells, the effect of Lipofectamine 2000-mediated transfection with APOC1 siRNA or an APOC1-overexpressing plasmid on cell growth and colony formation ability were examined by observing the growth curves and using colony-forming assay. The changes in cell cycle and apoptosis of the transfected cells were analyzed with flow cytometry. RT-qPCR and Western blotting were used to detect the changes in expressions of P21, P27, CDK4, cyclin D1, Bcl-2, Bax, caspase-3 and caspase-9 and the key proteins in the JAK2/STAT3 signaling pathway. RESULTS: APOC1 expression was significantly higher in PTC tissues and the 3 PTC cell lines than in the adjacent tissues and Nthyori 3-1 cells, respectively. In TPC-1 and BCPAP cells, APOC1 knockdown obviously reduced cell proliferative activity, increased the percentage of G0/G1 phase cells, lowered the percentages of S and G2 phase cells, promoted cell apoptosis, and downregulated mRNA and protein expression levels of CDK4, cyclin D1 and Bcl-2 and the protein levels of p-JAK2 and p-STAT3. APOC1 overexpression in the cells produced the opposite effects on cell proliferation, apoptosis, cell cycle and the mRNA and protein expressions. The application of AG490, a JAK2 inhibitor, strongly attenuated APOC1 overexpression-induced activation of the JAK2/STAT3 signaling pathway in BCPAP cells. CONCLUSIONS APOC1 overexpression promotes proliferation and inhibits apoptosis of PTC cells possibly by activating the JAK2/STAT3 signaling pathway and accelerating cell cycle progression.
Humans ; Apoptosis ; Cell Proliferation ; STAT3 Transcription Factor/metabolism* ; Signal Transduction ; Janus Kinase 2/metabolism* ; Thyroid Neoplasms/pathology* ; Thyroid Cancer, Papillary ; Cell Line, Tumor ; Carcinoma, Papillary

OBJECTIVE: To explore the rules of acupoint selection and compatibility of acupuncture and moxibustion in treatment of chronic cough using data mining. METHODS: The ancient and modern medical record cloud platform, and the databases, i.e. CNKI, Wanfang, VIP, EMbase, Web of Science and PubMed, were searched to screen the ancient and modern literature on acupuncture and moxibustion treatment of chronic cough. The prescription database was established for acupuncture and moxibustion treatment of chronic cough, and the analysis conducted on the frequency and use percentage in the aspects of intervention measures, acupoint selection, acupoint distribution, meridian tropism, special points and acupoint combination, as well as the association rules and clustering rules of acupoint selection. The subgroup analysis was performed in accordance with the etiology of chronic cough and intervention measures. RESULTS: A total of 106 articles were included and 158 prescriptions were extracted. The intervention measures were acupuncture, moxibustion, herbal medication and the combination of several measures. The high-frequency acupoints included Feishu (BL13), Zusanli (ST36), Dazhui (GV14), Pishu (BL20), Danzhong (CV17), Shenshu (BL23), Lieque (LU7), Dingchuan (EX-B1), Tiantu (CV22), and Fenglong (ST40). These acupoints are mainly distributed on the back, lumbar region, chest and abdomen. The involved meridians were bladder meridian of foot-taiyang, conception vessel, and lung meridian of hand-taiyin. The special points covered back-shu points, crossing points and five-shu point. Regarding the compatibility of acupoints, the combination of upper and lower points, and the combination of front and back points were predominant in treatment. The analysis of association rules found that the support of Feishu (BL13)→Zusanli (ST36) was the highest; the cluster analysis obtained 8 clusters of acupoints. The acupoint compatibility and overall rules were similar when cough variant asthma (CVA) or the mixed reasons were involved, and the local treatment approach was adopted if the etiology of disease was related to upper airway cough syndrome (UACS) and gastroesophageal reflux cough (GERC). The acupoint selection was similar among different intervention measures. When two kinds of measures were combined in treatment, Feishu (BL13), Pishu (BL20) and Zusanli (ST36) were the most common. CONCLUSION In treatment with acupuncture and moxibustion for chronic cough, the acupoints are selected on the affected local area, depending on syndrome differentiation, and focusing on back-shu points. The main acupoints are Feishu (BL13), Zusanli (ST36), Dazhui (GV14), Pishu (BL20), Danzhong (CV17) and Shenshu (BL23). The combined therapy is dominant with acupuncture, moxibustion and herbal medicine involved.
Acupuncture Points ; Moxibustion/history* ; Humans ; Cough/history* ; Acupuncture Therapy/history* ; Chronic Disease/therapy* ; Data Mining ; History, Ancient ; Meridians ; Chronic Cough

Objective:To evaluate the safety and effectiveness for the treatment of totally robotic fundoplication.Methods:A retrospective analysis was conducted on the clinical data of 21 patients with gastroesophageal reflux disease (GERD) who underwent unassisted totally robotic fundoplication at the Second Department of General Surgery, Yan'an Hospital Affiliated to Kunming Medical University from Aug 2023 to Jan 2024. The postoperative outcomes were evaluated using SF-36, GERD-Q, and NRS scoring indicators.Results:All 21 patients successfully underwent the surgery. The robotic surgery time was (99±41) minutes, with precise intraoperative anatomy and insignificant blood loss of (1.7±1.4) ml. There were no intraoperative or postoperative complications, and no conversions to open surgery . Postoperative recovery of bowel function was rapid (11.71±3.33) hours, with minimal postoperative pain (NRS score of 1.67±0.48).The postoperative hospital stay was short (3.86±2.90) days, and patient satisfaction was high, SF-36 score of (80.90±1.14);The symptoms of reflux after surgery was significantly reduced.Postoperative GERD-Q score of (4.38±1.69) significantly lower than the preoperative score of (13.90±2.07).Conclusion:Totally robotic fundoplication provides clear view of intraoperative anatomical structures, rapid postoperative recovery, minimal pain, and effective anti-reflux outcomes.

Objective:To investigate the effect and mechanism of the V-set and immunoglobulin domain-containing 4 ( VSIG4 ) gene mutation on the function of microglia in retinitis pigmentosa (RP). Methods:Localization of VSIG4 in the retina was detected by immunofluorescence.HMC3 cells (human microglial cells) were transfected with wild-type (Len-WT) VSIG4 gene, mutant type (Len-Mut) VSIG4 gene and empty vector virus (Len-Cont) and stimulated by the presence or absence of lipopolysaccharide (LPS), then divided into control group, LPS-Len-Mut group, LPS-Len-WT group, LPS-Len-Cont group, Len-Mut group, Len-WT group and Len-Cont group.The mRNA expression levels of the inflammatory factors interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were detected by real-time fluorescence quantitative PCR.Protein expression levels of nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase 1 (HO-1), glutathione peroxidase 4 (GPX4), nuclear transcription factor-κB (NF-κB) p65 subunit (P65), and phosphorylated P65 (PP65) were detected by Western blot.Cell phagocytic function was detected by phagocytosis assay.Cell migration ability was detected by cell scratch and transwell migration assay.LPS- stimulated HMC3 cells were co-cultured with 661W cells (mouse retinal photoreceptor cells), and the expression levels of B-cell lymphoma-2 (Bcl-2) and Bcl-2-associated X (Bax) proteins of the cells were detected by Western blot.The number of apoptotic cells was determined by apoptosis assay. Results:VSIG4 was localized to microglia in mouse retina.The real-time fluorescence quantitative PCR results showed that compared with LPS-Len-WT group, the relative expression levels of IL-1β and TNF-α mRNA in HMC3 cells were significantly increased in LPS-Len-Mut group (both at P<0.05).The Western blot results showed that compared with LPS- Len-WT group, the protein expression levels of Nrf2, HO-1, and GPX4 in HMC3 cells were significantly reduced in LPS-Len-Mut group, and the PP65/P65 ratio was significantly increased (all at P<0.05).The phagocytic experiment results showed that the phagocytic rates of HMC3 cells in Len-Cont group, LPS-Len-Cont group, LPS-Len-WT group, and LPS-Len-Mut group were (35.67±3.22)%, (63.67±10.07)%, (84.00±3.46)%, and (64.67±2.31)%, respectively, showing a statistically significant difference ( F=59.06, P<0.001).Compared with LPS-Len-WT group, the phagocytic rate of HMC3 cells was significantly reduced in LPS-Len-Mut group ( P<0.05).The results of cell scratch and transwell migration assay showed that compared with LPS-Len-WT group, the migration rate of HMC3 cells at 24 and 48 hours and the number of invading cells per unit area at 24 hours were significantly reduced in LPS-Len-Mut group (all at P<0.05).Compared with LPS-Len-WT group, the expression ratio of Bax/Bcl-2 protein and the number of cell apoptosis were significantly increased in the LPS-Len-Mut group under the co-culture system (both at P<0.05). Conclusions:VSIG4 is localized to mouse retinal microglia.When the VSIG4 gene in RP mutates, HMC3 cells under LPS stimulation exhibit a series of changes, including activation of the NF-κB signaling pathway, decreased activation of the Nrf2/HO-1 signaling pathway, increased secretion of inflammatory cytokines, reduced phagocytic and migratory abilities, and increased cell apoptosis in co-culture systems.

Objective To systematically evaluate the efficacy and safety of finerenone in treating the pa-tients with heart failure.Methods The databases of Pubmed,Embase,Cochrane Library,Web of Science and Scopus were retrieved.The retrieval time was from the establishment of the database to April 21,2024.The data of randomized controlled trials(RCTs)on finerenone in treating heart failure were collected.After screening the literatures and extracting the data,the Jadad scale and Cochrane bias risk assessment tool were used to evaluate the quality of included literatures.The RevMan5.4 software was adopted to conduct the meta analysis.Results Five RCTs involving in a total of 2 518 patients with heart failure were finally included.In the aspect of effectiveness outcome indicators,there was no statistical difference in improving NT-proBNP lev-els and cardiovascular mortality risk between finerenone and eplerenone(P＞0.05).Compared with placebo,finerenone could reduce the ris k of first hospitalization due to heart failure(RR=0.68,95％CI:0.49-0.94,P=0.02)and the risk of cardiovascular composite endpoint event(RR=0.79,95％CI:0.64-0.98,P=0.03).In the aspects of safe outcome indicators,the occurrence risk of adverse events of finerenone was slight-ly lower than that of placebo(RR=0.95,95％CI:0.90-1.01),the risk of finerenone induced hyperkalemia was slightly lower than that of eplerenone(RR=0.90,95％CI:0.46-1.76),but the difference was not statis-tically significant(P＞0.05).Finerenone had a higher risk for causing hyperkalemia than placebo(RR=2.07,95％CI:1.46-2.95,P＜0.01).Conclusion Finerenone could reduce the NT-proBNP level,risk of first time HHF and the cardiovascular composite endpoint event,moreover its safe and tolerance are good.

Objective To clarify the bottlenecks in the technology transfer work of public hospitals in China,and try to propose work optimization strategies and suggestions from the perspectives of different stakeholders.Methods The relevant literature about the transformation of scientific and technological achievements in public hospitals was searched on CNKI,and inductively summarize the relevant concepts and categories through the three stages of grounded theory coding.Results Through the three stages of coding,85 initial concepts,29 categories,and 5 main categories were sorted out,and a model attributing the influencing factors of the transformation of scientific and technological achievements in public hospitals was constructed,involving five major entities:public hospitals,medi-cal staff,enterprises,government,and universities.Conclusion The work of transforming scientific and technologi-cal achievements in public hospitals involves multiple stakeholders.It can start from the standardized management of public hospitals,the technological breakthroughs of medical staff,the active leadership of the government,the pro-active integration of enterprises,and the talent cultivation of universities to achieve innovation in talent cultivation mechanisms,industry evaluation mechanisms,profit distribution mechanisms,and intelligent management mecha-nisms.

Objective Understanding the cognitive attitude,influencing factors,and demand elements of medical staff in public hospitals towards the transformation of scientific and technological achievements,and proposing coun-termeasures and suggestions to improve the transformation rate of scientific and technological achievements in pub-lic hospitals.Methods Based on a questionnaire survey of the cognition and needs for the transformation of scientific and technological achievements among medical staff in public hospitals,it investigates medical personnel from 12 public hospitals in Anhui Province.Data processing is conducted using descriptive analysis and moderated mediation effect analysis methods.Results The cognitive attitude is positively correlated with influencing factors(r=0.125,P＜0.01),the degree of cognition is positively correlated with demand elements(r=0.599,P＜0.01),and influencing factors are positively correlated with demand elements(r=0.336,P＜0.01).The impact factors of medical staff in the process of scientific and technological achievement transformation play a partial mediating role between cognitive attitude and demand elements,and the technical title of medical staff has a moderating effect.Conclusion There are bottlenecks in the transformation of scientific and technological achievements by medical staff in public hospitals,in-cluding a weak awareness of transformation,limited transformation capabilities,disconnection between scientific re-search achievements and the market,and an unobvious collaborative effect of"government,education,medical care,production,and research".Public hospitals should improve supporting measures in aspects such as talent cultivation,management systems,professional training,and information construction.

Objective To evaluate the transformation of scientific and technological achievements in 12 affiliated hospitals of a medical university in Anhui Province,and to provide suggestions for the transformation of scientific and technological achievements in affiliated hospitals of colleges and universities.Methods According to the collected data,entropy weight method was used to assign weights to the index system,and TOPSIS method and rank sum ratio method were used for comprehensive evaluation.Results Among theevaluation indicators,the top 3 weights are the total amount of incoming accounts converted by self-implementation(4.801%),the total amount of incoming accounts earned by cooperating with others(4.801%),and the number of R&D institutions,transfer institutions,and conversion service platforms jointly built with affiliated universities(4.779%).In the fuzzy joint results,the top three hospitals were A,C and G,and the bottom three hospitals were K,H and E.There are obvious differences among hospitals,and there are differences between directly affiliated hospitals and non-directly affiliated hospitals.Conclusion The affiliated hospitals of colleges and universities should explore a development path of the transformation of scientific and technological achievements with their own characteristics and make the transformation from the actual situation.Transform the achievements into the discipline construction,deepen and expand the scientific research advantages;construct transformation consciousness raising system,and promote action with consciousness raising;colleges and universities lead the integration of medical industry to build a new ecology of medical production,study and research.

Objective To clarify the bottlenecks in the technology transfer work of public hospitals in China,and try to propose work optimization strategies and suggestions from the perspectives of different stakeholders.Methods The relevant literature about the transformation of scientific and technological achievements in public hospitals was searched on CNKI,and inductively summarize the relevant concepts and categories through the three stages of grounded theory coding.Results Through the three stages of coding,85 initial concepts,29 categories,and 5 main categories were sorted out,and a model attributing the influencing factors of the transformation of scientific and technological achievements in public hospitals was constructed,involving five major entities:public hospitals,medi-cal staff,enterprises,government,and universities.Conclusion The work of transforming scientific and technologi-cal achievements in public hospitals involves multiple stakeholders.It can start from the standardized management of public hospitals,the technological breakthroughs of medical staff,the active leadership of the government,the pro-active integration of enterprises,and the talent cultivation of universities to achieve innovation in talent cultivation mechanisms,industry evaluation mechanisms,profit distribution mechanisms,and intelligent management mecha-nisms.

Objective Understanding the cognitive attitude,influencing factors,and demand elements of medical staff in public hospitals towards the transformation of scientific and technological achievements,and proposing coun-termeasures and suggestions to improve the transformation rate of scientific and technological achievements in pub-lic hospitals.Methods Based on a questionnaire survey of the cognition and needs for the transformation of scientific and technological achievements among medical staff in public hospitals,it investigates medical personnel from 12 public hospitals in Anhui Province.Data processing is conducted using descriptive analysis and moderated mediation effect analysis methods.Results The cognitive attitude is positively correlated with influencing factors(r=0.125,P＜0.01),the degree of cognition is positively correlated with demand elements(r=0.599,P＜0.01),and influencing factors are positively correlated with demand elements(r=0.336,P＜0.01).The impact factors of medical staff in the process of scientific and technological achievement transformation play a partial mediating role between cognitive attitude and demand elements,and the technical title of medical staff has a moderating effect.Conclusion There are bottlenecks in the transformation of scientific and technological achievements by medical staff in public hospitals,in-cluding a weak awareness of transformation,limited transformation capabilities,disconnection between scientific re-search achievements and the market,and an unobvious collaborative effect of"government,education,medical care,production,and research".Public hospitals should improve supporting measures in aspects such as talent cultivation,management systems,professional training,and information construction.